LC-MS/MS-based metabolite profiling and functional assessment of Hydrodictyon reticulatum as a potential source of natural therapeutics collected from the Yamuna River, India
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Date
2026-04-28
Authors
Kumar, Dharmendra
Agrawal, Shivakar
Prajapati, Manisha
Sahoo, Sanjukta
Sahoo, Dinabandhu
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier B.V.
Published Version
Abstract
Hydrodictyon , a filamentous green alga, was found across multiple sites in the Yamuna River, India, during COVID-19, forming harmful algal blooms. Its potential uses remain underexplored. This study investigates the nutraceutical, antioxidant, and antidiabetic properties of Hydrodictyon reticulatum collected from different locations. The metabolic analysis revealed the highest concentrations of carbohydrates in Haryana (24.29 ± 0.12%), reducing sugars in Baghpat (8.41 ± 0.22%), chlorophyll a in Baghpat (0.30 ± 0.06 mg/g DW), chlorophyll b in Baghpat and Katha (0.49 ± 0.08/0.02 mg/g DW), carotenoids in Baghpat (0.39 ± 0.01 mg/g DW), and flavonoids in Baghpat (13.07 ± 0.73 mg QE/g DW). The sample from Khekra exhibited the highest protein content (5.85 ± 0.13%) and phenolic content (117.57 ± 3.94 mg GAE/g DW). Lipid content, relevant for biodiesel applications, peaked in Haryana (31.47 ± 0.87%). The strongest antioxidant responses were observed for DPPH in Katha (86.45 ± 3.73%), TAC in Katha (411.80 ± 4.23 mg TAE/g DW), and FRAP in Katha (16.97 ± 0.55 µmol TE/g DW). For antidiabetic activity, the lowest (strongest) α-glucosidase IC₅₀ was recorded in Katha (0.09 ± 0.03 mg/ml), while the lowest α-amylase IC₅₀ occurred in Khekra (0.13 ± 0.01 mg/ml). LC-MS/MS analysis revealed a diverse array of bioactive compounds, including lipids, sterols, flavonoids, and phenolic acids. Combined LC-MS/MS, FTIR, and CHNS analyses highlight the antioxidant, anti-inflammatory, nutraceutical, and biodiesel potential of H. reticulatum from the Yamuna River.
Description
Keywords
Antioxidants , CHNS , FTIR , LCMS/MS , Lipid , Metabolomics , Principal component analysis (PCA) , [APCMicrobiome] , α-Amylase , α-Glucosidase
Citation
Kumar, D, Agrawal, S, Prajapati, M, Sahoo, S & Sahoo, D 2026, 'LC-MS/MS-based metabolite profiling and functional assessment of Hydrodictyon reticulatum as a potential source of natural therapeutics collected from the Yamuna River, India', Applied Food Research, vol. 6, no. 1, 102062, pp. 1-17. https://doi.org/10.1016/j.afres.2026.102062