Correlation between pre-treatment quasispecies complexity and treatment outcome in chronic HCV genotype 3a

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dc.contributor.authorMoreau, Isabelle
dc.contributor.authorLevis, John
dc.contributor.authorCrosbie, Orla
dc.contributor.authorKenny-Walsh, Elizabeth
dc.contributor.authorFanning, Liam J.
dc.contributor.funderRoche Pharmaceuticals, Irelanden
dc.date.accessioned2016-06-17T14:10:09Z
dc.date.available2016-06-17T14:10:09Z
dc.date.issued2008-07-09
dc.date.updated2013-01-18T15:07:01Z
dc.description.abstractPre-treatment HCV quasispecies complexity and diversity may predict response to interferon based anti-viral therapy. The objective of this study was to retrospectively (1) examine temporal changes in quasispecies prior to the start of therapy and (2) investigate extensively quasispecies evolution in a group of 10 chronically infected patients with genotype 3a, treated with pegylated alpha 2a-Interferon and ribavirin. The degree of sequence heterogeneity within the hypervariable region 1 was assessed by analyzing 20-30 individual clones in serial serum samples. Genetic parameters, including amino acid Shannon entropy, Hamming distance and genetic distance were calculated for each sample. Treatment outcome was divided into (1) sustained virological responders (SVR) and (2) treatment failure (TF).Our results indicate, (1) quasispecies complexity and diversity are lower in the SVR group, (2) quasispecies vary temporally and (3) genetic heterogeneity at baseline can be used to predict treatment outcome. We discuss the results from the perspective of replicative homeostasis. We discuss the results from the perspective of replicative homeostasis.en
dc.description.sponsorshipRoche Pharmaceuticals, Ireland (unrestricted research grant)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid78
dc.identifier.citationMoreau, I., Levis, J., Crosbie, O., Kenny-Walsh, E. and Fanning, L. J. (2008) 'Correlation between pre-treatment quasispecies complexity and treatment outcome in chronic HCV genotype 3a', Virology Journal, 5: 78. http://dx.doi.org/10.1186/1743-422X-5-78en
dc.identifier.doi10.1186/1743-422X-5-78
dc.identifier.endpage15en
dc.identifier.issn1743-422X
dc.identifier.journaltitleVirology Journalen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/2755
dc.identifier.volume5en
dc.language.isoenen
dc.publisherBiomed Centralen
dc.rights© 2008 Moreau et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citeden
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/en
dc.subjectHepatitis C virusen
dc.subjectInterferon-alpha-2b plus ribavirinen
dc.subjectHypervariable region 1en
dc.subjectAntiviral therapyen
dc.subjectReplicative homeostasisen
dc.subjectPegylated interferonen
dc.subjectSequence variabilityen
dc.subjectGenetic diversityen
dc.subjectImmune selectionen
dc.subjectEnvelope proteinen
dc.titleCorrelation between pre-treatment quasispecies complexity and treatment outcome in chronic HCV genotype 3aen
dc.typeArticle (peer-reviewed)en
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