Bioconjugated gold nanoparticles enhance siRNA delivery in prostate cancer cells

dc.contributor.authorRahme, Kamil
dc.contributor.authorGuo, Jianfeng
dc.contributor.authorHolmes, Justin D.
dc.contributor.editorDinesh Kumar, Lekha
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderIrish Research Councilen
dc.contributor.funderDepartment of Science and Technology of Jilin Provinceen
dc.contributor.funderNational Council for Scientific Researchen
dc.date.accessioned2019-05-22T15:51:03Z
dc.date.available2019-05-22T15:51:03Z
dc.date.issued2019-05-17
dc.date.updated2019-05-22T09:23:05Z
dc.description.abstractHere we describe a simple way to create a gold nanoparticle (AuNP)-based non-viral delivery system to deliver siRNA into prostate cancer cells. Therefore, positively charged polyethylenimine (PEI)-capped AuNPs were synthesized in water and further conjugated with the targeting ligand (folic acid) for folate receptors (AuNPs-PEI-FA). The AuNPs-PEI-FA could effectively complex small interfering RNA (siRNA) through electrostatic interaction. Flow cytometry displayed that AuNPs-PEI-FA could specifically deliver siRNA into LNCaP cells, a prostate cancer cell line overexpressing prostate-specific membrane antigen (PSMA) that exhibits a hydrolase enzymatic activity with a folate substrate. In contrast, internalization of siRNA into PC-3 cells, a prostate cancer cell line not expressing PSMA or folate receptors, was not achieved using AuNPs-PEI-FA.siRNA. Following endolysosomal escape, the AuNPs-PEI-FA-.siRNA formulation resulted in significant endogenous gene silencing when compared to the nontargeted formulation, suggesting the potential of AuNPs-PEI-FA for targeted delivery of therapeutic siRNAs in the treatment of prostate cancer.en
dc.description.sponsorshipIrish Research Council (Government of Ireland Postdoctoral Fellowship (GOIPD/2013/150)); Department of Science and Technology of Jilin Province (Outstanding Youth Foundation from the Department of Science and Technology, Jilin Province (Project Number: 20170520046JH)); National Council for Scientific Research, Lebanon (CNRS-L-GRP2015-3538).en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRahme, K., Guo, J. and Holmes, J. D. (2019) 'Bioconjugated Gold Nanoparticles Enhance siRNA Delivery in Prostate Cancer Cells', in Dinesh Kumar, L. (ed.) RNA Interference and Cancer Therapy: Methods and Protocols. New York, NY: Springer New York, pp. 291-301. doi: 10.1007/978-1-4939-9220-1_21en
dc.identifier.doi10.1007/978-1-4939-9220-1_21en
dc.identifier.endpage301en
dc.identifier.journaltitleRNA Interference and Cancer Therapy: Methods and Protocolsen
dc.identifier.startpage291en
dc.identifier.urihttps://hdl.handle.net/10468/7970
dc.language.isoenen
dc.publisherSpringeren
dc.relation.ispartofRNA Interference and Cancer Therapy: Methods and Protocols, Methods in Molecular Biology
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2278/IE/Advanced Materials and BioEngineering Research Centre (AMBER)/en
dc.relation.urihttps://link.springer.com/protocol/10.1007%2F978-1-4939-9220-1_21
dc.rights© 2019 Springer. This is a post-peer-review, pre-copyedit version of a book chapter published in RNA Interference and Cancer Therapy Methods and Protocols; Methods in Molecular Biology book series (MIMB, volume 1974), The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-9220-1_21en
dc.subjectGold nanoparticlesen
dc.subjectTargeting ligandsen
dc.subjectReceptor-mediated internalizationen
dc.subjectNon-viral siRNA deliveryen
dc.subjectProstate canceren
dc.subjectGene therapyen
dc.titleBioconjugated gold nanoparticles enhance siRNA delivery in prostate cancer cellsen
dc.typeBook chapteren
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