Bioconjugated gold nanoparticles enhance siRNA delivery in prostate cancer cells

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dc.contributor.authorRahme, Kamil
dc.contributor.authorGuo, Jianfeng
dc.contributor.authorHolmes, Justin D.
dc.contributor.editorDinesh Kumar, Lekha
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderIrish Research Councilen
dc.contributor.funderDepartment of Science and Technology of Jilin Provinceen
dc.contributor.funderNational Council for Scientific Researchen
dc.date.accessioned2019-05-22T15:51:03Z
dc.date.available2019-05-22T15:51:03Z
dc.date.issued2019-05-17
dc.date.updated2019-05-22T09:23:05Z
dc.description.abstractHere we describe a simple way to create a gold nanoparticle (AuNP)-based non-viral delivery system to deliver siRNA into prostate cancer cells. Therefore, positively charged polyethylenimine (PEI)-capped AuNPs were synthesized in water and further conjugated with the targeting ligand (folic acid) for folate receptors (AuNPs-PEI-FA). The AuNPs-PEI-FA could effectively complex small interfering RNA (siRNA) through electrostatic interaction. Flow cytometry displayed that AuNPs-PEI-FA could specifically deliver siRNA into LNCaP cells, a prostate cancer cell line overexpressing prostate-specific membrane antigen (PSMA) that exhibits a hydrolase enzymatic activity with a folate substrate. In contrast, internalization of siRNA into PC-3 cells, a prostate cancer cell line not expressing PSMA or folate receptors, was not achieved using AuNPs-PEI-FA.siRNA. Following endolysosomal escape, the AuNPs-PEI-FA-.siRNA formulation resulted in significant endogenous gene silencing when compared to the nontargeted formulation, suggesting the potential of AuNPs-PEI-FA for targeted delivery of therapeutic siRNAs in the treatment of prostate cancer.en
dc.description.sponsorshipIrish Research Council (Government of Ireland Postdoctoral Fellowship (GOIPD/2013/150)); Department of Science and Technology of Jilin Province (Outstanding Youth Foundation from the Department of Science and Technology, Jilin Province (Project Number: 20170520046JH)); National Council for Scientific Research, Lebanon (CNRS-L-GRP2015-3538).en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationRahme, K., Guo, J. and Holmes, J. D. (2019) 'Bioconjugated Gold Nanoparticles Enhance siRNA Delivery in Prostate Cancer Cells', in Dinesh Kumar, L. (ed.) RNA Interference and Cancer Therapy: Methods and Protocols. New York, NY: Springer New York, pp. 291-301. doi: 10.1007/978-1-4939-9220-1_21en
dc.identifier.doi10.1007/978-1-4939-9220-1_21en
dc.identifier.endpage301en
dc.identifier.journaltitleRNA Interference and Cancer Therapy: Methods and Protocolsen
dc.identifier.startpage291en
dc.identifier.urihttps://hdl.handle.net/10468/7970
dc.language.isoenen
dc.publisherSpringeren
dc.relation.ispartofRNA Interference and Cancer Therapy: Methods and Protocols, Methods in Molecular Biology
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2278/IE/Advanced Materials and BioEngineering Research Centre (AMBER)/en
dc.relation.urihttps://link.springer.com/protocol/10.1007%2F978-1-4939-9220-1_21
dc.rights© 2019 Springer. This is a post-peer-review, pre-copyedit version of a book chapter published in RNA Interference and Cancer Therapy Methods and Protocols; Methods in Molecular Biology book series (MIMB, volume 1974), The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-4939-9220-1_21en
dc.subjectGold nanoparticlesen
dc.subjectTargeting ligandsen
dc.subjectReceptor-mediated internalizationen
dc.subjectNon-viral siRNA deliveryen
dc.subjectProstate canceren
dc.subjectGene therapyen
dc.titleBioconjugated gold nanoparticles enhance siRNA delivery in prostate cancer cellsen
dc.typeBook chapteren
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