Shining light on food microbiology; applications of luciferase-tagged microorganisms in the food industry

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dc.check.embargoformatE-thesis on CORA onlyen
dc.check.entireThesisEntire Thesis Restricted
dc.check.opt-outNoen
dc.check.reasonThis thesis is due for publication or the author is actively seeking to publish this materialen
dc.contributor.advisorHill, Colinen
dc.contributor.advisorBegley, Máireen
dc.contributor.authorMorrissey, Ruth M.
dc.contributor.funderDepartment of Agriculture, Food and the Marine, Irelanden
dc.date.accessioned2014-02-19T14:37:13Z
dc.date.available2015-02-20T05:00:05Z
dc.date.issued2013
dc.date.submitted2013
dc.description.abstractBioluminescence is the production of light by living organisms as a result of a number of enzyme catalysed reactions caused by enzymes termed luciferases. The lux genes responsible for the emission of light can be cloned from one bioluminescent microorganism into one that is not bioluminescent. The light emitted can be monitored and quantified and will provide information on the metabolic activity, quantity and location of cells in a particular environment, in real-time. The primary aim of this thesis was to investigate and identify several food industry related applications of lux-tagged microorganisms. The first aim was to monitor a lux-tagged Cronobacter sakazakii in reconstituted infant milk formula, in realtime. The second aim was to investigate a bioluminescent-based early warning system for starter culture disruption by bacteriophages and antibiotic residues. The third of this thesis was to examine the use of a bioluminescent-based assay to test the activity of bioengineered Nisin derivatives M21V and S29A against foodborne pathogens in laboratory media and selected foods.en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine, Ireland (Food Institutional Research Measure (08RDC602))en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMorrissey, R. M. 2013. Shining light on food microbiology; applications of luciferase-tagged microorganisms in the food industry. PhD Thesis, University College Cork.en
dc.identifier.endpage182
dc.identifier.urihttps://hdl.handle.net/10468/1395
dc.language.isoenen
dc.publisherUniversity College Corken
dc.rights© 2013, Ruth M. Morrisseyen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectLuciferaseen
dc.subject.lcshLuciferasesen
dc.subject.lcshBioluminescenceen
dc.subject.lcshFood--Microbiologyen
dc.thesis.opt-outfalse
dc.titleShining light on food microbiology; applications of luciferase-tagged microorganisms in the food industryen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD (Science)en
ucc.workflow.supervisorc.hill@ucc.ie
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