The dissemination of C10 cysteine protease genes in Bacteroides fragilis species by mobile genetic elements

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dc.contributor.authorThornton, Roibeard F.
dc.contributor.authorKagawa, Todd F.
dc.contributor.authorO'Toole, Paul W.
dc.contributor.authorCooney, Jakki C.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderUniversity of Limericken
dc.contributor.funderDepartment of Agriculture, Fisheries and Food, Irelanden
dc.contributor.funderWellcome Trust, United Kingdomen
dc.date.accessioned2012-11-21T15:45:09Z
dc.date.available2012-11-21T15:45:09Z
dc.date.copyright2010
dc.date.issued2010-04-23
dc.description.abstractBackground: The C10 family of cysteine proteases includes enzymes that contribute to the virulence of bacterial pathogens, such as SpeB in Streptococcus pyogenes. The presence of homologues of cysteine protease genes in human commensal organisms has not been examined. Bacteroides fragilis is a member of the dominant Bacteroidetes phylum of the human intestinal microbiota, and is a significant opportunistic pathogen. Results: Four homologues of the streptococcal virulence factor SpeB were identified in the B. fragilis genome. These four protease genes, two were directly contiguous to open reading frames predicted to encode staphostatin-like inhibitors, with which the protease genes were co-transcribed. Two of these protease genes are unique to B. fragilis 638R and are associated with two large genomic insertions. Gene annotation indicated that one of these insertions was a conjugative Tn-like element and the other was a prophage-like element, which was shown to be capable of excision. Homologues of the B. fragilis C10 protease genes were present in a panel of clinical isolates, and in DNA extracted from normal human faecal microbiota. Conclusions: This study suggests a mechanism for the evolution and dissemination of an important class of protease in major members of the normal human microbiota.en
dc.description.sponsorshipScience Foundation Ireland (08/RFP/BMT1596); Science Foundation Ireland (SFI-CSET); University of Limerick (UL PhD Studentship); Government of Ireland Dept of Agriculture Fisheries and Food (DAFF FHRI)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationThornton, R, T.F. Kagawa, P.W. O’Toole and J.C. Cooney. 2010. The dissemination of C10 cysteine protease genes in Bacteroides fragilis species by mobile genetic elements. BMC Microbiol. 10: 122. DOI: 10.1186/1471-2180-10-122en
dc.identifier.doi10.1186/1471-2180-10-122
dc.identifier.issn1471-2180
dc.identifier.issued10en
dc.identifier.journaltitleBMC Microbiologyen
dc.identifier.startpage122en
dc.identifier.urihttps://hdl.handle.net/10468/793
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urihttp://www.biomedcentral.com/1471-2180/10/122
dc.rights© 2010 Thornton et al; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en
dc.subjectC10en
dc.subjectCysteine Proteasesen
dc.subjectStreptococcus pyogenesen
dc.subjectBacteroides fragilisen
dc.subjectSpeBen
dc.subjectHuman Microbiotaen
dc.titleThe dissemination of C10 cysteine protease genes in Bacteroides fragilis species by mobile genetic elementsen
dc.typeArticle (peer-reviewed)en
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