The CWPS Rubik's cube: Linking diversity of cell wall polysaccharide structures with the encoded biosynthetic machinery of selected Lactococcus lactis strains

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dc.contributor.authorMahony, Jennifer
dc.contributor.authorFrantzen, Cyril
dc.contributor.authorVinogradov, Evgeny
dc.contributor.authorSadovskaya, Irina
dc.contributor.authorTheodorou, Ilias
dc.contributor.authorKelleher, Philip
dc.contributor.authorChapot-Chartier, Marie-Pierre
dc.contributor.authorCambillau, Christian
dc.contributor.authorHolo, Helge
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderNorges Forskningsråden
dc.contributor.funderTINEen
dc.date.accessioned2021-11-15T14:10:18Z
dc.date.available2021-11-15T14:10:18Z
dc.date.issued2020-06-09
dc.date.updated2021-11-15T13:55:18Z
dc.description.abstractThe biosynthetic machinery for cell wall polysaccharide (CWPS) production in lactococci is encoded by a large gene cluster, designatedcwps. This locus displays considerable variation among lactococcal genomes, previously prompting a classification into three distinct genotypes (A-C). In the present study, thecwpsloci of 107 lactococcal strains were compared, revealing the presence of a fourthcwpsgenotype (type D). Lactococcal CWPSs are comprised of two saccharidic structures: a peptidoglycan-embedded rhamnan backbone polymer to which a surface-exposed, poly/oligosaccharidic side-chain is covalently linked. Chemical structures of the side-chain of seven lactococcal strains were elucidated, highlighting their diverse and strain-specific nature. Furthermore, a link betweencwpsgenotype and chemical structure was derived based on the number of glycosyltransferase-encoding genes in thecwpscluster and the presence of conserved genes encoding the presumed priming glycosyltransferase. This facilitates predictions of several structural features of lactococcal CWPSs including (a) whether the CWPS possesses short oligo/polysaccharide side-chains, (b) the number of component monosaccharides in a given CWPS structure, (c) the order of monosaccharide incorporation into the repeating units of the side-chain (for C-type strains), (d) the presence of Galfand phosphodiester bonds in the side-chain, and (e) the presence of glycerol phosphate substituents in the side-chain.en
dc.description.sponsorshipScience Foundation Ireland (SFI/12/RC/2273-P1; SFI/12/RC/2273-P2); Norges Forskningsråd (Grant number 225246)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMahony, J., Frantzen, C., Vinogradov, E., Sadovskaya, I., Theodorou, I., Kelleher, P., Chapot-Chartier, M.-P., Cambillau, C., Holo, H. and van Sinderen, D. (2020) 'The CWPS Rubik's cube: Linking diversity of cell wall polysaccharide structures with the encoded biosynthetic machinery of selected Lactococcus lactis strains', Molecular Microbiology, 114(4), pp. 582-596. doi: 10.1111/mmi.14561en
dc.identifier.doi10.1111/mmi.14561en
dc.identifier.eissn1365-2958
dc.identifier.endpage596en
dc.identifier.issn0950-382X
dc.identifier.issued4en
dc.identifier.journaltitleMolecular Microbiologyen
dc.identifier.startpage582en
dc.identifier.urihttps://hdl.handle.net/10468/12217
dc.identifier.volume114en
dc.language.isoenen
dc.publisherJohn Wiley & Sons, Inc.en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/en
dc.rights© 2020, John Wiley & Sons Ltd. This is the peer reviewed version of the following item: Mahony, J., Frantzen, C., Vinogradov, E., Sadovskaya, I., Theodorou, I., Kelleher, P., Chapot-Chartier, M.-P., Cambillau, C., Holo, H. and van Sinderen, D. (2020) 'The CWPS Rubik's cube: Linking diversity of cell wall polysaccharide structures with the encoded biosynthetic machinery of selectedLactococcus lactisstrains', Molecular Microbiology, 114(4), pp. 582-596, doi: 10.1111/mmi.14561, which has been published in final form at: https://doi.org/10.1111/mmi.14561 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en
dc.subjectLactococcus-lactisen
dc.subjectReceptoren
dc.subjectIdentificationen
dc.subjectAlgorithmen
dc.titleThe CWPS Rubik's cube: Linking diversity of cell wall polysaccharide structures with the encoded biosynthetic machinery of selected Lactococcus lactis strainsen
dc.typeArticle (peer-reviewed)en
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