Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal

Simple item page
dc.contributor.authorJames, Kieran
dc.contributor.authorBottacini, Francesca
dc.contributor.authorContreras, Jose Ivan Serrano
dc.contributor.authorVigoureux, Mariane
dc.contributor.authorEgan, Muireann
dc.contributor.authorMotherway, Mary O’Connell
dc.contributor.authorHolmes, Elaine
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderMizutani Foundation for Glycoscienceen
dc.contributor.funderIrish Research Councilen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHealth Research Boarden
dc.contributor.funderFederation of European Microbiological Societiesen
dc.contributor.funderEuropean Society of Clinical Microbiology and Infectious Diseasesen
dc.contributor.funderNational Institute for Health Researchen
dc.contributor.funderNIHR Imperial Biomedical Research Centreen
dc.date.accessioned2019-12-04T10:30:53Z
dc.date.available2019-12-04T10:30:53Z
dc.date.issued2019-10-28
dc.description.abstractA number of bifidobacterial species are found at a particularly high prevalence and abundance in faecal samples of healthy breastfed infants, a phenomenon that is believed to be, at least partially, due to the ability of bifidobacteria to metabolize Human Milk Oligosaccharides (HMOs). In the current study, we isolated a novel strain of Bifidobacterium kashiwanohense, named APCKJ1, from the faeces of a four-week old breastfed infant, based on the ability of the strain to utilise the HMO component fucosyllactose. We then determined the full genome sequence of this strain, and employed the generated data to analyze fucosyllactose metabolism in B. kashiwanohense APCKJ1. Transcriptomic and growth analyses, combined with metabolite analysis, in vitro hydrolysis assays and heterologous expression, allowed us to elucidate the pathway for fucosyllactose metabolism in B. kashiwanohense APCKJ1. Homologs of the key genes for this metabolic pathway were identified in particular in infant-derived members of the Bifdobacterium genus, revealing the apparent niche-specific nature of this pathway, and allowing a broad perspective on bifidobacterial fucosyllactose and L-fucose metabolism.en
dc.description.sponsorshipMizutani Foundation for Glycoscience (ref no. 190049); Irish Research Council (ID GOIPG/2013/651); HRB (Grant No. PDTM/20011/9); FEMS Research Grant (FEMS-RG-2016-0103)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid15427en
dc.identifier.citationJames, K., Bottacini, F., Contreras, J. I. S., Vigoureux, M., Egan, M., Motherway, M. O. c., Holmes, E. and van Sinderen, D. (2019) 'Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal', Scientific Reports, 9(1), 15427. (20pp.) doi: 10.1038/s41598-019-51901-7en
dc.identifier.doi10.1038/s41598-019-51901-7en
dc.identifier.eissn2045-2322
dc.identifier.endpage20en
dc.identifier.issued1en
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9313
dc.identifier.volume9en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.urihttps://www.nature.com/articles/s41598-020-73762-1
dc.rights© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBacterial genomicsen
dc.subjectCellular microbiologyen
dc.subjectMetabolismen
dc.titleMetabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensalen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 3 of 3
Thumbnail Image
Name:
s41598-019-51901-7.pdf
Size:
3.92 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download
Thumbnail Image
Name:
41598_2019_51901_MOESM1_ESM.pdf
Size:
1.29 MB
Format:
Adobe Portable Document Format
Description:
Supplementary file 1
Download
Thumbnail Image
Name:
s41598-020-73762-1.pdf
Size:
683.05 KB
Format:
Adobe Portable Document Format
Description:
Author Correction
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles