A novel cold active esterase from a deep sea sponge Stelletta normani metagenomic library

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dc.contributor.authorBorchert, Erik
dc.contributor.authorSelvin, Joseph
dc.contributor.authorKiran, Seghal G.
dc.contributor.authorJackson, Stephen A.
dc.contributor.authorO'Gara, Fergal
dc.contributor.authorDobson, Alan D. W.
dc.contributor.funderMarine Institute
dc.contributor.funderSeventh Framework Programme
dc.date.accessioned2017-10-18T09:40:15Z
dc.date.available2017-10-18T09:40:15Z
dc.date.issued2017
dc.description.abstractEsterases catalyze the hydrolysis of ester bonds in fatty acid esters with short-chain acyl groups. Due to the widespread applications of lipolytic enzymes in various industrial applications, there continues to be an interest in novel esterases with unique properties. Marine ecosystems have long been acknowledged as a significant reservoir of microbial biodiversity and in particular of bacterial enzymes with desirable characteristics for industrial use, such as for example cold adaptation and activity in the alkaline pH range. We employed a functional metagenomic approach to exploit the enzymatic potential of one particular marine ecosystem, namely the microbiome of the deep sea sponge Stelletta normani. Screening of a metagenomics library from this sponge resulted in the identification of a number of lipolytic active clones. One of these encoded a highly, cold-active esterase 7N9, and the recombinant esterase was subsequently heterologously expressed in Escherichia coli. The esterase was classified as a type IV lipolytic enzyme, belonging to the GDSAG subfamily of hormone sensitive lipases. Furthermore, the recombinant 7N9 esterase was biochemically characterized and was found to be most active at alkaline pH (8.0) and displays salt tolerance over a wide range of concentrations. In silico docking studies confirmed the enzyme's activity toward short-chain fatty acids while also highlighting the specificity toward certain inhibitors. Furthermore, structural differences to a closely related mesophilic E40 esterase isolated from a marine sediment metagenomics library are discussed.en
dc.description.sponsorshipMarine Institute (Beaufort Marine Research Award (Sea Change Strategy and the Strategy for Science Technology and Innovation (2006–2012); Marine Research Sub-Programme of the National Development Plan 2007–2013).en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid287
dc.identifier.citationBorchert, E., Selvin, J., Kiran, S. G., Jackson, S. A., O'Gara, F. and Dobson, A. D. W. (2017) 'A novel cold active esterase from a deep sea sponge Stelletta normani metagenomic library', Frontiers in Marine Science, 4, 287 (13pp). doi: 10.3389/fmars.2017.00287en
dc.identifier.doi10.3389/fmars.2017.00287
dc.identifier.endpage13
dc.identifier.issn2296-7745
dc.identifier.journaltitleFrontiers in Marine Scienceen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/4898
dc.identifier.volume4
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmars.2017.00287/full
dc.rights© 2017, Borchert, Selvin, Kiran, Jackson, O'Gara and Dobson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these termsen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCold active esteraseen
dc.subjectMetagenomic libraryen
dc.subjectDeep sea spongeen
dc.titleA novel cold active esterase from a deep sea sponge Stelletta normani metagenomic libraryen
dc.typeArticle (peer-reviewed)en
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