Different carbon sources result in differential activation of sigma B and stress resistance in Listeria monocytogenes

Simple item page
dc.contributor.authorCrespo Tapia, Natalia
dc.contributor.authorDorey, Amber L.
dc.contributor.authorGahan, Cormac G. M.
dc.contributor.authorden Besten, Heidy M. W.
dc.contributor.authorO'Byrne, Conor P.
dc.contributor.authorAbee, Tjakko
dc.contributor.funderHorizon 2020en
dc.date.accessioned2020-01-10T12:14:30Z
dc.date.available2020-01-10T12:14:30Z
dc.date.issued2019-12-30
dc.date.updated2020-01-07T12:37:09Z
dc.description.abstractListeria monocytogenes is an important food-borne pathogen that is ubiquitous in the environment. It is able to utilize a variety of carbon sources, to produce biofilms on food-processing surfaces and to survive food preservation–associated stresses. In this study, we investigated the effect of three common carbon sources, namely glucose, glycerol and lactose, on growth and activation of the general stress response Sigma factor, SigB, and corresponding phenotypes including stress resistance. A fluorescent reporter coupled to the promoter of lmo2230, a highly SigB-dependent gene, was used to determine SigB activation via quantitative fluorescence spectroscopy. This approach, combined with Western blotting and fluorescence microscopy, showed the highest SigB activation in lactose grown cells and lowest in glucose grown cells. In line with this observation, lactose grown cells showed the highest resistance to lethal heat and acid stress, the highest biofilm formation, and had the highest adhesion/invasion capacity in Caco-2-derived C2Bbe1 cell lines. Our data suggest that lactose utilisation triggers a strong SigB dependent stress response and this may have implications for the resistance of L. monocytogenes along the food chain.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationCrespo Tapia, N., Dorey, A. L., Gahan, C. G. M., den Besten, H. M. W., O'Byrne, C. P. and Abee, T. (2019) 'Different carbon sources result in differential activation of sigma B and stress resistance in Listeria monocytogenes', International Journal of Food Microbiology. doi: 10.1016/j.ijfoodmicro.2019.108504en
dc.identifier.doi10.1016/j.ijfoodmicro.2019.108504en
dc.identifier.eissn1879-3460
dc.identifier.issn0168-1605
dc.identifier.journaltitleInternational Journal of Food Microbiologyen
dc.identifier.urihttps://hdl.handle.net/10468/9483
dc.language.isoenen
dc.publisherElsevier B.V.en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-ITN-ETN/641984/EU/Training and research in Listeria monocytogenes Adaptation through Proteomic and Transcriptome deep Sequencing Analysis/List_MAPSen
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S0168160519304350
dc.rights© 2019, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectCarbon sourceen
dc.subjectGlucoseen
dc.subjectGlycerolen
dc.subjectLactoseen
dc.subjectSigma Ben
dc.subjectSigBen
dc.subjectStresen
dc.subjectStress responseen
dc.subjectHeat stressen
dc.subjectAcid stressen
dc.subjectBiofilmen
dc.subjectVirulenceen
dc.subjectAdhesion assayen
dc.subjectInvasion assayen
dc.subjectC2Bbe1 cell lineen
dc.titleDifferent carbon sources result in differential activation of sigma B and stress resistance in Listeria monocytogenesen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1-s2.0-S0168160519304350-main.pdf
Size:
2.24 MB
Format:
Adobe Portable Document Format
Description:
Accepted Version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Microbiology - Journal Articles
APC Microbiome Ireland - Journal Articles