A bioengineered nisin derivative to control Streptococcus uberis biofilms

dc.contributor.authorPĂ©rez-Ibarreche, Marianaen
dc.contributor.authorField, Desen
dc.contributor.authorRoss, R. Paulen
dc.contributor.authorHill, Colinen
dc.contributor.funderHorizon 2020en
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2023-05-31T14:56:58Z
dc.date.available2023-05-31T14:56:58Z
dc.date.issued2021-07-27T00:00:00Zen
dc.description.abstractAntimicrobial peptides are evolving as novel therapeutic options against the increasing problem of multidrug-resistant microorganisms, and nisin is one such avenue. However, some bacteria possess a specific nisin resistance system (NSR), which cleaves the peptide reducing its bactericidal efficacy. NSR-based resistance was identified in strains of Streptococcus uberis, a ubiquitous pathogen that causes mastitis in dairy cattle. Previous studies have demonstrated that a nisin A derivative termed nisin PV, featuring S29P and I30V, exhibits enhanced resistance to proteolytic cleavage by NSR. Our objective was to investigate the ability of this nisin derivative to eradicate and inhibit biofilms of S. uberis DPC 5344 and ATCC 700407 () using crystal violet (biomass), 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) (viability) assays, and confocal microscopy (viability and architecture). When preestablished biofilms were assessed, both peptides reduced biofilm biomass by over 60% compared to that of the untreated controls. However, a 42% higher reduction in viability was observed following treatment with nisin PV compared to that of nisin A. Accordingly, confocal microscopy analysis revealed significantly more dead cells on the biofilm upper surface and a reduced thickness following treatment with nisin PV. When biofilm inhibition was assessed, nisin PV inhibited biofilm formation and decreased viability up to 56% and 85% more than nisin A, respectively. Confocal microscopy analysis revealed a lack of biofilm for ATCC 700407 and only dead cells for DPC 5344. These results suggest that nisin PV is a promising alternative to effectively reduce the biofilm formation of strains carrying NSR. One of the four most prevalent species of bovine mastitis-causing pathogens is Its ability to form biofilms confers on the bacteria greater resistance to antibiotics, requiring higher doses to be more effective. In a bid to limit antibiotic resistance development, the need for alternative antimicrobials is paramount. Bacteriocins such as nisin represent one such alternative that could alleviate the impact of mastitis caused by However, many strains of have been shown to possess nisin resistance determinants, such as the nisin resistance protein (NSR). In this study, we demonstrate the ability of nisin and a nisin derivative termed PV that is insensitive to NSR to prevent and remove biofilms of NSR-producing strains. These findings will add new information to the antimicrobial bacteriocins and control of research fields specifically in relation to biofilms and mastitis-associated strains.en
dc.description.sponsorshipScience Foundation Ireland (SFI/12/RC/2273 P2)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide00391-21en
dc.identifier.citationPĂ©rez-Ibarreche, M., Field, D., Ross, R. P. and Hill, C. (2021) 'A bioengineered nisin derivative to control Streptococcus uberis biofilms', Applied and Environmental Microbiology, 87(16), e00391-21 (14pp). doi: 10.1128/AEM.00391-21en
dc.identifier.doi10.1128/AEM.00391-21en
dc.identifier.eissn1098-5336en
dc.identifier.endpage14en
dc.identifier.issn0099-2240en
dc.identifier.issued16en
dc.identifier.journaltitleApplied and Environmental Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14533
dc.identifier.volume87en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-COFUND-FP/754535/EU/APC Postdoctoral EXcellence Programme/APEXen
dc.rights© 2021, Pérez-Ibarreche et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectNSRen
dc.subjectStreptococcus uberisen
dc.subjectAntimicrobial agentsen
dc.subjectBiofilmsen
dc.subjectNisin Aen
dc.subjectNisin PVen
dc.titleA bioengineered nisin derivative to control Streptococcus uberis biofilmsen
dc.typeArticle (peer-reviewed)en
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