Genomic characterization of sulphite reducing bacteria isolated from the dairy production chain

Simple item page
dc.contributor.authorDoyle, Conor J.
dc.contributor.authorO'Toole, Paul W.
dc.contributor.authorCotter, Paul D.
dc.contributor.funderTeagasc
dc.date.accessioned2018-08-29T15:47:25Z
dc.date.available2018-08-29T15:47:25Z
dc.date.issued2018
dc.description.abstractAnaerobic sporeformers, specifically spoilage and pathogenic members of the genus Clostridium, are a concern for producers of dairy products, and of powdered dairy products in particular.As an alternative to testing for individual species, the traditional, and still current, approach to detecting these sporeformers, including non-spoilage/non-pathogenic species, in dairy products has involved testing for a sulphite reducing phenotype [Sulphite reducing Clostridia (SRCs)] under anaerobic conditions. This phenotype is conserved throughout the Order Clostridia. Unfortunately, however, this phenotype is exhibited by other sulphite reducing bacteria (SRBs) also, potentially leading to potential for false positives. Here, this risk was borne out through the identification of several SRBs from industry samples that were identified as Proteus mirabilis and various Bacillus/Paenibacillus sp. Genome wide comparison of a number of representative SRCs and SRBs was employed to determine phylogenetic relationships, especially among SRCs, and to characterize the genes responsible for the sulphite reducing phenotype. This screen identified two associated operons, i.e., asrABC in SRCs, and cysJI in Bacillus/Paembacillus spp. and P. mirabilis. This screen identified spp. belonging to sensu stricto. Lachnospiraceae and Cluster XIV of the Clostridia all producing the SRC phenotype. This study highlights the inaccuracy of the industry standard SRC test but highlights the potential to generate an equivalent molecular test designed to detect the genes responsible for this phenotype in Clostridia.en
dc.description.sponsorshipTeagasc (2013030; RMIS6364)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1507
dc.identifier.citationDoyle, C. J., O'Toole, P. W. and Cotter, P. D. (2018) 'Genomic characterization of sulphite reducing bacteria isolated from the dairy production chain', Frontiers in Microbiology, 9, 1507 (9pp). doi: 10.3389/fmicb.2018.01507en
dc.identifier.doi10.3389/fmicb.2018.01507
dc.identifier.endpage9
dc.identifier.issn1664-302X
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/6671
dc.identifier.volume9
dc.language.isoenen
dc.publisherFrontiers Media S.A.en
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2018.01507/full
dc.rights© 2018, the Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSulphite reducing bacteriaen
dc.subjectSRCsen
dc.subjectSulphite reducing clostridiaen
dc.subjectGenomicsen
dc.subjectFood microbesen
dc.titleGenomic characterization of sulphite reducing bacteria isolated from the dairy production chainen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Genomic Characterization of.pdf
Size:
1.88 MB
Format:
Adobe Portable Document Format
Description:
Published Version
Download
Collections
APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles