Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats

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dc.check.date2017-12-09
dc.check.infoAccess to this item is restricted until 12 months after publication by the request of the publisher.en
dc.contributor.authorAhmeda, Ahmad F.
dc.contributor.authorRae, Mark G.
dc.contributor.authorAl Otaibi, Mohammed F.
dc.contributor.authorAnweigi, Lamyia M.
dc.contributor.authorJohns, Edward J.
dc.contributor.funderCollege of Medicine, King Saud Universityen
dc.date.accessioned2017-01-24T11:59:34Z
dc.date.available2017-01-24T11:59:34Z
dc.date.issued2016-12-09
dc.date.updated2017-01-10T13:49:12Z
dc.description.abstractVasoconstriction within the renal medulla contributes to the development of hypertension. This study investigated the role of reactive oxygen species (ROS) in regulating renal medullary and cortical blood perfusion (MBP and CBP respectively) in both stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar rats. CBP and MBP were measured using a laser-Doppler flow meter before and after intra-renal infusion of tempol, the superoxide dismutase (SOD) mimetic or tempol plus catalase, the hydrogen peroxide-degrading enzyme. Tempol infusion significantly elevated blood perfusion within the renal medulla (MBP) in both SHRSP (by 43 ± 7%, P < 0.001) and Wistar rats (by 17 ± 2%, P < 0.05) but the magnitude of the increase was significantly greater in the SHRSP (P < 0.01). When the enzyme catalase and tempol were co-infused, MBP was again significantly increased in SHRSP (by 57 ± 6%, P < 0.001) and Wistar rats (by 33 ± 6%, P < 0.001), with a significantly greater increase in perfusion being induced in the SHRSP relative to the Wistar rats (P < 0.01). Notably, this increase was significantly greater than in those animals infused with tempol alone (P < 0.01). These results suggest that ROS plays a proportionally greater role in reducing renal vascular compliance, particularly within the renal medulla, in normotensive and hypertensive animals, with effects being greater in the hypertensive animals. This supports the hypothesis that SHRSP renal vasculature might be subjected to elevated level of oxidative stress relative to normotensive animals.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAhmeda, A. F., Rae, M. G., Al Otaibi, M. F., Anweigi, L. M. and Johns, E. J. (2016) 'Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats', Journal of Physiology and Biochemistry, pp. 1-8. First Online. doi: 10.1007/s13105-016-0541-1en
dc.identifier.doi10.1007/s13105-016-0541-1
dc.identifier.journaltitleJournal Of Physiology And Biochemistryen
dc.identifier.urihttps://hdl.handle.net/10468/3508
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.rights© University of Navarra 2016. Published by Springer Verlag. The final publication is available at Springer via http://dx.doi.org/10.1007/s13105-016-0541-1en
dc.subjectRenal blood perfusionen
dc.subjectTempolen
dc.subjectCatalaseen
dc.subjectReactive oxygen speciesen
dc.subjectSHRSPen
dc.titleEffect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar ratsen
dc.typeArticle (peer-reviewed)en
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