Cystathione gamma lyase/hydrogen sulphide pathway up regulation enhances the responsiveness of ?1A and ?1B-adrenoreceptors in the kidney of rats with left ventricular hypertrophy

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dc.contributor.authorAhmad, Ashfaq
dc.contributor.authorSattar, Munavvar A.
dc.contributor.authorAzam, Maleeha
dc.contributor.authorAbdulla, Mohammed H.
dc.contributor.authorKhan, Safia A.
dc.contributor.authorHashmi, Fayyaz
dc.contributor.authorAbdullah, Nor A.
dc.contributor.authorJohns, Edward J.
dc.contributor.funderInstitute of Postgraduate Studies, Universiti Sains Malaysia
dc.contributor.funderUniversiti Sains Malaysia
dc.date.accessioned2017-06-21T11:01:26Z
dc.date.available2017-06-21T11:01:26Z
dc.date.issued2016-05-18
dc.description.abstractThe purpose of the present study was to investigate the interaction between H2S and NO (nitric oxide) in the kidney and to evaluate its impact on the functional contribution of ?1A and ?1B-adrenoreceptors subtypes mediating the renal vasoconstriction in the kidney of rats with left ventricular hypertrophy (LVH). In rats the LVH induction was by isoprenaline administration and caffeine in the drinking water together with intraperitoneal administration of H2S. The responsiveness of ?1A and ?1B to exogenous noradrenaline, phenylephrine and methoxaminein the absence and presence of 5-methylurapidil (5-MeU) and chloroethylclonidine (CEC) was studied. Cystathione gamma lyase (CSE), cystathione ? synthase (CBS), 3-mercaptopyruvate sulphar transferase (3-MST) and endothelial nitric oxide synthase (eNOS) were quantified. There was significant up regulation of CSE and eNOS in the LVH-H2S compared to the LVH group (P<0.05). Baseline renal cortical blood perfusion (RCBP) was increased (P<0.05) in the LVH-H2S compared to the LVH group. The responsiveness of ?1A-adrenergic receptors to adrenergic agonists was increased (P<0.05) after administration of low dose 5-Methylurapidil in the LVH-H2S group while ?1B-adrenergic receptors responsiveness to adrenergic agonists were increased (P<0.05) by both low and high dose chloroethylclonidine in the LVH-H2S group. Treatment of LVH with H2S resulted in up-regulation of CSE/H2S, CBS, and 3-MST and eNOS/NO/cGMP pathways in the kidney. These up regulation of CSE/H2S, CBS, and 3-MST and eNOS/NO/cGMP pathways enhanced the responsiveness of ?1A and ?1B-adrenoreceptors subtypes to adrenergic agonists in LVH-H2S. These findings indicate an important role for H2S in modulating deranged signalling in the renal vasculature resulting from LVH development.en
dc.description.sponsorshipInstitute of Postgraduate Studies (APEX (1002/JHEA/ATSG4001); Universiti Sains Malaysia (grant no.1001/PFARMASI/815078 and HIR grant UM.0000069/HIR.C3)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0154995
dc.identifier.citationAhmad, A., Sattar, M. A., Azam, M., Abdulla, M. H., Khan, S. A., Hashmi, F., Abdullah, N. A. and Johns, E. J. (2016) 'Cystathione gamma lyase/Hydrogen sulphide pathway up regulation enhances the responsiveness of α1A and α1B-adrenoreceptors in the kidney of rats with left ventricular hypertrophy', PLoS ONE, 11(5), e0154995 (23pp). doi: 10.1371/journal.pone.0154995en
dc.identifier.doi10.1371/journal.pone.0154995
dc.identifier.endpage23
dc.identifier.issn1932-6203
dc.identifier.issued5
dc.identifier.journaltitlePLoS ONEen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/4135
dc.identifier.volume11
dc.language.isoenen
dc.publisherPLoSen
dc.relation.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154995
dc.rights© 2016, Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectKidneysen
dc.subjectBlooden
dc.subjectVasoconstrictionen
dc.subjectRNA extractionen
dc.subjectRenal systemen
dc.subjectUrineen
dc.subjectVasodilationen
dc.subjectRenal physiologyen
dc.titleCystathione gamma lyase/hydrogen sulphide pathway up regulation enhances the responsiveness of ?1A and ?1B-adrenoreceptors in the kidney of rats with left ventricular hypertrophyen
dc.typeArticle (peer-reviewed)en
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