Three new Escherichia coli phages from the human gut show promising potential for phage therapy

Simple item page
dc.contributor.authorDalmasso, Marion
dc.contributor.authorStrain, Ronan
dc.contributor.authorNeve, Horst
dc.contributor.authorFranz, Charles M. A. P.
dc.contributor.authorCousin, Fabien J.
dc.contributor.authorRoss, R. Paul
dc.contributor.authorHill, Colin
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2017-06-21T11:01:26Z
dc.date.available2017-06-21T11:01:26Z
dc.date.issued2016-06-09
dc.description.abstractWith the emergence of multi-drug resistant bacteria the use of bacteriophages (phages) is gaining renewed interest as promising anti-microbial agents. The aim of this study was to isolate and characterize phages from human fecal samples. Three new coliphages, ?APCEc01, ?APCEc02 and ?APCEc03, were isolated. Their phenotypic and genomic characteristics, and lytic activity against biofilm, and in combination with ciprofloxacin, were investigated. All three phages reduced the growth of E. coli strain DPC6051 at multiplicity of infection (MOI) between 10?3 and 105. A cocktail of all three phages completely inhibited the growth of E. coli. The phage cocktail also reduced biofilm formation and prevented the emergence of phage-resistant mutants which occurred with single phage. When combined with ciprofloxacin, phage alone or in cocktail inhibited the growth of E. coli and prevented the emergence of resistant mutants. These three new phages are promising biocontrol agents for E. coli infections.en
dc.description.sponsorshipScience Foundation Ireland (Grant Number SFI/12/RC/2273)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0156773
dc.identifier.citationDalmasso, M., Strain, R., Neve, H., Franz, C. M. A. P., Cousin, F. J., Ross, R. P. and Hill, C. (2016) 'Three new Escherichia coli phages from the human gut show promising potential for phage therapy', PLoS ONE, 11(6), e0156773 (16pp). doi:en
dc.identifier.doi10.1371/journal.pone.0156773
dc.identifier.endpage16
dc.identifier.issn1932-6203
dc.identifier.issued6
dc.identifier.journaltitlePLoS ONEen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/4133
dc.identifier.volume11
dc.language.isoenen
dc.publisherPLoSen
dc.relation.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0156773
dc.rights© 2016, Dalmasso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriophagesen
dc.subjectEscherichia coli infectionsen
dc.subjectBacterial biofilmsen
dc.subjectViral genomicsen
dc.subjectAntibioticsen
dc.subjectComparative genomicsen
dc.subjectBird genomicsen
dc.subjectGene predictionen
dc.titleThree new Escherichia coli phages from the human gut show promising potential for phage therapyen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 4 of 4
Thumbnail Image
Name:
3006.PDF
Size:
1.62 MB
Format:
Adobe Portable Document Format
Description:
Published Version
Download
Thumbnail Image
Name:
3006-1.XLSX
Size:
340.35 KB
Format:
Microsoft Excel XML
Description:
Supplementary File 1
Download
Thumbnail Image
Name:
3006-2.XLSX
Size:
272.07 KB
Format:
Microsoft Excel XML
Description:
Supplementary File 2
Download
Thumbnail Image
Name:
3006-3.XLSX
Size:
187.81 KB
Format:
Microsoft Excel XML
Description:
Supplementary File 3
Download
Collections
Microbiology - Journal Articles
APC Microbiome Ireland - Journal Articles