Impact of bacteriocin-producing strains on bacterial community composition in a simplified human intestinal microbiota

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dc.contributor.authorRíos Colombo, Natalia S.en
dc.contributor.authorPérez-Ibarreche, Marianaen
dc.contributor.authorDraper, Lorraine A.en
dc.contributor.authorO’Connor, Paula M.en
dc.contributor.authorField, Desen
dc.contributor.authorRoss, R. Paulen
dc.contributor.authorHill, Colinen
dc.contributor.funderHorizon 2020en
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Research Councilen
dc.date.accessioned2024-04-17T14:29:09Z
dc.date.available2024-04-17T14:29:09Z
dc.date.issued2023en
dc.description.abstractBacteriocins are antimicrobial peptides that have been studied for decades as food bio-preservatives or as alternatives to antibiotics. They also have potential as modulators of the gut microbiome, which has been linked to human health. However, it is difficult to predict a priori how bacteriocins will impact complex microbial communities through direct and indirect effects. Here we assess the effect of different bacteriocin-producing strains on a Simplified Human Intestinal Microbiota (SIHUMI) model, using a set of bacteriocin-producing strains (Bac+) and otherwise isogenic non-producers (Bac−). Bacteriocins from different classes and with different activity spectra were selected, including lantibiotics such as lacticin 3147 and nisin A, and pediocin-like bacteriocins such as pediocin PA-1 among other peptides. SIHUMI is a bacterial consortium of seven diverse human gut species that assembles to a predictable final composition in a particular growth medium. Each member can be individually tracked by qPCR. Bac+ and Bac− strains were superimposed on the SIHUMI system, and samples were taken at intervals up to 48 h. The genome copy number of each SIHUMI member was evaluated using specific primers. We establish that the composition of the community changes in response to the presence of either broad- or narrow-spectrum bacteriocin producers and confirm that there are significant off-target effects. These effects were analyzed considering antagonistic inter-species interactions within the SIHUMI community, providing a comprehensive insight into the possible mechanisms by which complex communities can be shaped by bacteriocins.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1290697en
dc.identifier.citationRíos Colombo, N.S., Perez-Ibarreche, M., Draper, L.A., O’Connor, P.M., Field, D., Ross, R.P. and Hill, C. (2023) ‘Impact of bacteriocin-producing strains on bacterial community composition in a simplified human intestinal microbiota’, Frontiers in Microbiology, 14, 1290697 (14pp). doi: 10.3389/fmicb.2023.1290697en
dc.identifier.doi10.3389/fmicb.2023.1290697en
dc.identifier.eissn1664-302Xen
dc.identifier.endpage14en
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15808
dc.identifier.volume14en
dc.language.isoenen
dc.publisherFrontiers Media S.A.en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-IF-EF-ST/101027450/EU/Bacteriocins Impact on Obesity and MicrobiotA/BIOMAen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/HE::ERC::HORIZON-ERC/101054719/EU/Bacteriocins from interbacterial warfare as antibiotic alternative/BACtheWINNERen
dc.rights© 2023 Ríos Colombo, Perez-Ibarreche, Draper, O’Connor, Field, Ross and Hill. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriocinen
dc.subjectPediocinen
dc.subjectLantibioticen
dc.subjectLacticinen
dc.subjectNisinen
dc.subjectMicrobiomeen
dc.subjectIntestinal microbiotaen
dc.subjectSIHUMen
dc.titleImpact of bacteriocin-producing strains on bacterial community composition in a simplified human intestinal microbiotaen
dc.typeArticle (peer-reviewed)en
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