Nisin J, a novel natural nisin variant, is produced by Staphylococcus capitis sourced from the human skin microbiota

Simple item page
dc.contributor.authorO'Sullivan, Julie N.
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorRea, Mary C.
dc.contributor.authorO'Sullivan, Orla
dc.contributor.authorWalsh, Calum J.
dc.contributor.authorHealy, Brian
dc.contributor.authorMathur, Harsh
dc.contributor.authorField, Des
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2023-03-03T12:12:33Z
dc.date.available2023-03-03T12:12:33Z
dc.date.issued2020-01
dc.date.updated2023-03-03T11:54:44Z
dc.description.abstractThe skin microbiota is thought to play a key role in host protection from infection. Nisin J is a novel nisin variant produced by Staphylococcus capitis APC 2923, a strain isolated from the toe web space area in a screening study performed on the human skin microbiota. Whole-genome sequencing and mass spectrometry of the purified peptide confirmed that S. capitis APC 2923 produces a 3,458-Da bacteriocin, designated nisin J, which exhibited antimicrobial activity against a range of Gram-positive pathogens, including methicillin-resistant Staphylococcus aureus (MRSA) and Cutibacterium acnes The gene order in the nisin J gene cluster (nsjFEGBTCJP) differs from that of other nisin variants in that it is lacking the nisin regulatory genes, nisRK, as well as the nisin immunity gene nisI Nisin J has 9 amino acid changes compared to prototypical nisin A, with 8 amino acid substitutions, 6 of which are not present in other nisin variants (Ile4Lys, Met17Gln, Gly18Thr, Asn20Phe, Met21Ala, Ile30Gly, Val33His, and Lys34Thr), and an extra amino acid close to the C terminus, rendering nisin J the only nisin variant to contain 35 amino acids. This is the first report of a nisin variant produced by a Staphylococcus species and the first nisin producer isolated from human skin.IMPORTANCE This study describes the characterization of nisin J, the first example of a natural nisin variant, produced by a human skin isolate of staphylococcal origin. Nisin J displays inhibitory activity against a wide range of bacterial targets, including MRSA. This work demonstrates the potential of human commensals as a source for novel antimicrobials that could form part of the solution to antibiotic resistance across a broad range of bacterial pathogens.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationO’Sullivan, J. N., O’Connor, P. M., Rea, M. C., O’Sullivan, O., Walsh, C. J., Healy, B., Mathur, H., Field, D., Hill, C. and Ross, R. P. (2020) ‘Nisin J, a novel natural nisin variant, is produced by Staphylococcus capitis sourced from the human skin microbiota’, Journal of Bacteriology, 202(3), (15pp). doi: 10.1128/JB.00639-19en
dc.identifier.doi10.1128/JB.00639-19en
dc.identifier.endpage15en
dc.identifier.issn1098-5530
dc.identifier.issued3en
dc.identifier.journaltitleJournal of Bacteriologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14276
dc.identifier.volume202en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2020, O’Sullivan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAntimicrobial agentsen
dc.subjectAntimicrobial peptidesen
dc.subjectAntimicrobial structureen
dc.subjectBacteriocinsen
dc.subjectMicrobiotaen
dc.subjectNatural antimicrobial productsen
dc.subjectNisinen
dc.subjectSkin microbiotaen
dc.titleNisin J, a novel natural nisin variant, is produced by Staphylococcus capitis sourced from the human skin microbiotaen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
JB.00639-19.pdf
Size:
2.92 MB
Format:
Adobe Portable Document Format
Description:
Published Version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles