Transcriptional frameshifting rescues Citrobacter rodentium Type VI secretion by the production of two length variants from the prematurely interrupted tssM gene
dc.contributor.author | Gueguen, Erwan | |
dc.contributor.author | Wills, Norma M. | |
dc.contributor.author | Atkins, John F. | |
dc.contributor.author | Cascales, Eric | |
dc.contributor.funder | Centre National de la Recherche Scientifique, France | fr |
dc.contributor.funder | Aix-Marseille Université, France | fr |
dc.contributor.funder | Agence Nationale de la Recherche | fr |
dc.contributor.funder | Science Foundation Ireland | en |
dc.contributor.funder | Fondation pour la Recherche Médicale, France | fr |
dc.date.accessioned | 2016-02-17T11:43:38Z | |
dc.date.available | 2016-02-17T11:43:38Z | |
dc.date.issued | 2014 | |
dc.description.abstract | The Type VI secretion system (T6SS) mediates toxin delivery into both eukaryotic and prokaryotic cells. It is composed of a cytoplasmic structure resembling the tail of contractile bacteriophages anchored to the cell envelope through a membrane complex composed of the TssL and TssM inner membrane proteins and of the TssJ outer membrane lipoprotein. The C-terminal domain of TssM is required for its interaction with TssJ, and for the function of the T6SS. In Citrobacter rodentium, the tssM1 gene does not encode the C-terminal domain. However, the stop codon is preceded by a run of 11 consecutive adenosines. In this study, we demonstrate that this poly-A tract is a transcriptional slippery site that induces the incorporation of additional adenosines, leading to frameshifting, and hence the production of two TssM1 variants, including a full-length canonical protein. We show that both forms of TssM1, and the ratio between these two forms, are required for the function of the T6SS in C. rodentium. Finally, we demonstrate that the tssM gene associated with the Yersinia pseudotuberculosis T6SS-3 gene cluster is also subjected to transcriptional frameshifting. | en |
dc.description.sponsorship | Agence National de la Recherché, France (ANR-10-JCJC-1303-03); Fondation pour la Recherche Médicale, France (SPF-2009-12-17571) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Published Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.articleid | e1004869 | |
dc.identifier.citation | Gueguen E, Wills NM, Atkins JF, Cascales E (2014) Transcriptional Frameshifting Rescues Citrobacter rodentium Type VI Secretion by the Production of Two Length Variants from the Prematurely Interrupted tssM Gene. PLoS Genet 10(12): e1004869. doi:10.1371/journal.pgen.1004869 | |
dc.identifier.doi | 10.1371/journal.pgen.1004869 | |
dc.identifier.issn | 1553-7390 | |
dc.identifier.issued | 12 | en |
dc.identifier.journaltitle | PLOS GENETICS | en |
dc.identifier.uri | https://hdl.handle.net/10468/2319 | |
dc.identifier.volume | 10 | en |
dc.language.iso | en | en |
dc.publisher | Public Library of Science | en |
dc.rights | © 2015 Gueguen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | Enteroaggregative Escherichia coli | en |
dc.subject | Interbacterial competition | en |
dc.subject | Agrobacterium tumefaciens | en |
dc.subject | Translational frameshift | en |
dc.subject | Shigella flexneri | en |
dc.subject | Target cells | en |
dc.subject | System | en |
dc.subject | Protein | en |
dc.subject | Slippage | en |
dc.subject | Effectors | en |
dc.title | Transcriptional frameshifting rescues Citrobacter rodentium Type VI secretion by the production of two length variants from the prematurely interrupted tssM gene | en |
dc.type | Article (peer-reviewed) | en |
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