Transcriptional frameshifting rescues Citrobacter rodentium Type VI secretion by the production of two length variants from the prematurely interrupted tssM gene

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dc.contributor.authorGueguen, Erwan
dc.contributor.authorWills, Norma M.
dc.contributor.authorAtkins, John F.
dc.contributor.authorCascales, Eric
dc.contributor.funderCentre National de la Recherche Scientifique, Francefr
dc.contributor.funderAix-Marseille Université, Francefr
dc.contributor.funderAgence Nationale de la Recherchefr
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderFondation pour la Recherche Médicale, Francefr
dc.date.accessioned2016-02-17T11:43:38Z
dc.date.available2016-02-17T11:43:38Z
dc.date.issued2014
dc.description.abstractThe Type VI secretion system (T6SS) mediates toxin delivery into both eukaryotic and prokaryotic cells. It is composed of a cytoplasmic structure resembling the tail of contractile bacteriophages anchored to the cell envelope through a membrane complex composed of the TssL and TssM inner membrane proteins and of the TssJ outer membrane lipoprotein. The C-terminal domain of TssM is required for its interaction with TssJ, and for the function of the T6SS. In Citrobacter rodentium, the tssM1 gene does not encode the C-terminal domain. However, the stop codon is preceded by a run of 11 consecutive adenosines. In this study, we demonstrate that this poly-A tract is a transcriptional slippery site that induces the incorporation of additional adenosines, leading to frameshifting, and hence the production of two TssM1 variants, including a full-length canonical protein. We show that both forms of TssM1, and the ratio between these two forms, are required for the function of the T6SS in C. rodentium. Finally, we demonstrate that the tssM gene associated with the Yersinia pseudotuberculosis T6SS-3 gene cluster is also subjected to transcriptional frameshifting.en
dc.description.sponsorshipAgence National de la Recherché, France (ANR-10-JCJC-1303-03); Fondation pour la Recherche Médicale, France (SPF-2009-12-17571)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide1004869
dc.identifier.citationGueguen E, Wills NM, Atkins JF, Cascales E (2014) Transcriptional Frameshifting Rescues Citrobacter rodentium Type VI Secretion by the Production of Two Length Variants from the Prematurely Interrupted tssM Gene. PLoS Genet 10(12): e1004869. doi:10.1371/journal.pgen.1004869
dc.identifier.doi10.1371/journal.pgen.1004869
dc.identifier.issn1553-7390
dc.identifier.issued12en
dc.identifier.journaltitlePLOS GENETICSen
dc.identifier.urihttps://hdl.handle.net/10468/2319
dc.identifier.volume10en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2015 Gueguen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectEnteroaggregative Escherichia colien
dc.subjectInterbacterial competitionen
dc.subjectAgrobacterium tumefaciensen
dc.subjectTranslational frameshiften
dc.subjectShigella flexnerien
dc.subjectTarget cellsen
dc.subjectSystemen
dc.subjectProteinen
dc.subjectSlippageen
dc.subjectEffectorsen
dc.titleTranscriptional frameshifting rescues Citrobacter rodentium Type VI secretion by the production of two length variants from the prematurely interrupted tssM geneen
dc.typeArticle (peer-reviewed)en
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