Bacterial microcompartment-mediated ethanolamine metabolism in E. coli urinary tract infection

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dc.contributor.authorDadswell, Katherine
dc.contributor.authorCreagh, Sinead
dc.contributor.authorMcCullagh, Edward
dc.contributor.authorLiang, Mingzhi
dc.contributor.authorBrown, Ian R.
dc.contributor.authorWarren, Martin J. McNally, Alan
dc.contributor.authorMacSharry, John
dc.contributor.authorPrentice, Michael B.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen
dc.date.accessioned2019-06-05T09:14:37Z
dc.date.available2019-06-05T09:14:37Z
dc.date.issued2019-05-28
dc.date.updated2019-06-05T09:06:52Z
dc.description.abstractUrinary tract infections (UTIs) are common, in general caused by intestinal Uropathogenic E. coli (UPEC) ascending via the urethra. Microcompartment-mediated catabolism of ethanolamine, a host cell breakdown product, fuels competitive overgrowth of intestinal E. coli, both pathogenic enterohaemorrhagic E. coli and commensal strains. During UTI urease negative E. coli thrive, despite the comparative nutrient limitation in urine. The role of ethanolamine as a potential nutrient source during UTI is understudied. We evaluated the role of metabolism of ethanolamine as a potential nitrogen and carbon source for UPEC in the urinary tract. We analysed infected urine samples by culture, HPLC, qRT-PCR and genomic sequencing. Ethanolamine concentration in urine was comparable to the most abundant reported urinary amino acid D-serine. Transcription of the eut operon was detected in the majority of urine samples screened containing E. coli. All sequenced UPECs had conserved eut operons while metabolic genotypes previously associated with UTI (dsdCXA, metE) were mainly limited to phylogroup B2. In vitro ethanolamine was found to be utilised as a sole source of nitrogen by UPECs. Metabolism of ethanolamine in artificial urine medium (AUM) induced metabolosome formation and provided a growth advantage at the physiological levels found in urine. Interestingly, eutE (acetaldehyde dehydrogenase) was required for UPECs to utilise ethanolamine to gain a growth advantage in AUM, suggesting ethanolamine is also utilised as a carbon source. This data suggests urinary ethanolamine is a significant additional carbon and nitrogen source for infecting E. coli.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleidIAI.00211-19en
dc.identifier.citationDadswell, K., Creagh, S., McCullagh, E., Liang, M., Brown, I. R., Warren, M. J., McNally, A., MacSharry, J. and Prentice, M. B. (2019) 'Bacterial microcompartment-mediated ethanolamine metabolism in E. coli urinary tract infection', Infection and Immunity, IAI.00211-19. In Press, doi: 10.1128/iai.00211-19en
dc.identifier.doi10.1128/iai.00211-19en
dc.identifier.eissn1098-5522
dc.identifier.endpage47en
dc.identifier.issn0019-9567
dc.identifier.journaltitleInfection and Immunityen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8014
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/BBSRC/BB/M002969/1/GB/Development of supramolecular assemblies for enhancing cellular productivity and the synthesis of fine chemicals and biotherapeutics./en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/BBSRC/BB/H013180/1/GB/Synthetic biology approaches to compartmentalisation in bacteria and the construction of novel bioreactors/en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/BBSRC/BB/L024209/1/GB/MicrobesNG: A scalable replicable biological sample repository incorporating whole-genome sequence data and analysis of thousands of microbial strains/en
dc.relation.urihttps://iai.asm.org/content/iai/early/2019/05/21/IAI.00211-19.full.pdf
dc.rights© 2019 Dadswell et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMicrocompartmenten
dc.subjectMetabolosomeen
dc.subjectUrinary tract infectionen
dc.subjectE. colien
dc.subjectEthanolamineen
dc.titleBacterial microcompartment-mediated ethanolamine metabolism in E. coli urinary tract infectionen
dc.typeArticle (peer-reviewed)en
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