Redox biology response in germinating Phaseolus vulgaris seeds exposed to copper: evidence for differential redox buffering in seedlings and cotyledon
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Date
2017
Authors
Karmous, Ines
Trevisan, Rafael
El Ferjani, Ezzedine
Chaoui, Abdelilah
Sheehan, David
Journal Title
Journal ISSN
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Publisher
Public Library of Science
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Abstract
In agriculture, heavy metal contamination of soil interferes with processes associated with plant growth, development and productivity. Here, we describe oxidative and redox changes, and deleterious injury within cotyledons and seedlings caused by exposure of germinating (Phaseolus vulgaris L. var. soisson nain hatif) seeds to copper (Cu). Cu induced a marked delay in seedling growth, and was associated with biochemical disturbances in terms of intracellular oxidative status, redox regulation and energy metabolism. In response to these alterations, modulation of activities of antioxidant proteins (thioredoxin and glutathione reductase, peroxiredoxin) occurred, thus preventing oxidative damage. In addition, oxidative modification of proteins was detected in both cotyledons and seedlings by one- and two-dimensional electrophoresis. These modified proteins may play roles in redox buffering. The changes in activities of redox proteins underline their fundamental roles in controlling redox homeostasis. However, observed differential redox responses in cotyledon and seedling tissues showed a major capacity of the seedlings' redox systems to protect the reduced status of protein thiols, thus suggesting quantitatively greater antioxidant protection of proteins in seedlings compared to cotyledon. To our knowledge, this is the first comprehensive redox biology investigation of the effect of Cu on seed germination.
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Keywords
Induced oxidative stress , Superoxide dismutase , Hydrogen peroxide , Thioredoxin , Photosynthetic organisms , Antioxidative enzymes , Glutathione cycle , Abiotic stresses , Plant-responses , Pisum-sativum
Citation
Karmous, I., Trevisan, R., El Ferjani, E., Chaoui, A. and Sheehan, D. (2017) 'Redox biology response in germinating Phaseolus vulgaris seeds exposed to copper: evidence for differential redox buffering in seedlings and cotyledon', PLOS ONE, 12(10), e0184396 (23pp). doi: 10.1371/journal.pone.0184396