Early gut microbiota perturbations following intrapartum antibiotic prophylaxis to prevent group B streptococcal disease

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dc.contributor.authorMazzola, Giuseppe
dc.contributor.authorMurphy, Kiera
dc.contributor.authorRoss, R. Paul
dc.contributor.authorDi Gioia, Diana
dc.contributor.authorBiavati, Bruno
dc.contributor.authorCorvaglia, Luigi T.
dc.contributor.authorFaldella, Giacomo
dc.contributor.authorStanton, Catherine
dc.contributor.funderDepartment of Agriculture, Food and the Marine
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderGlobal Grant Spinner
dc.contributor.funderTeagasc
dc.contributor.funderSeventh Framework Programme
dc.date.accessioned2017-06-21T11:01:26Z
dc.date.available2017-06-21T11:01:26Z
dc.date.issued2016-06-22
dc.description.abstractThe faecal microbiota composition of infants born to mothers receiving intrapartum antibiotic prophylaxis with ampicillin against group B Streptococcus was compared with that of control infants, at day 7 and 30 of life. Recruited newborns were both exclusive breastfed and mixed fed, in order to also study the effect of dietary factors on the microbiota composition. Massive parallel sequencing of the V3-V4 region of the 16S rRNA gene and qPCR analysis were performed. Antibiotic prophylaxis caused the most marked changes on the microbiota in breastfed infants, mainly resulting in a higher relative abundance of Enterobacteriaceae, compared with control infants (52% vs. 14%, p = 0.044) and mixed-fed infants (52% vs. 16%, p = 0.13 NS) at day 7 and in a lower bacterial diversity compared to mixed-fed infants and controls. Bifidobacteria were also particularly vulnerable and abundances were reduced in breastfed (p = 0.001) and mixed-fed antibiotic treated groups compared to non-treated groups. Reductions in bifidobacteria in antibiotic treated infants were also confirmed by qPCR. By day 30, the bifidobacterial population recovered and abundances significantly increased in both breastfed (p = 0.025) and mixed-fed (p = 0.013) antibiotic treated groups, whereas Enterobacteriaceae abundances remained highest in the breastfed antibiotic treated group (44%), compared with control infants (16%) and mixed-fed antibiotic treated group (28%). This study has therefore demonstrated the short term consequences of maternal intrapartum antibiotic prophylaxis on the infant faecal microbial population, particularly in that of breastfed infants.en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine (INFANTMET Project (RefNo 10FDairy); Science Foundation of Ireland (funded Centre for Science, Engineering and Technology, the Alimentary Pharmabiotic Centre); Global Grant Spinner (project (2013); Teagasc (Walsh Fellowship)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0157527
dc.identifier.citationMazzola, G., Murphy, K., Ross, R. P., Di Gioia, D., Biavati, B., Corvaglia, L. T., Faldella, G. and Stanton, C. (2016) 'Early Gut Microbiota Perturbations Following Intrapartum Antibiotic Prophylaxis to Prevent Group B Streptococcal Disease', PLOS ONE, 11(6), e0157527 (11pp). doi:en
dc.identifier.doi10.1371/journal.pone.0157527
dc.identifier.endpage11
dc.identifier.issn1932-6203
dc.identifier.issued6
dc.identifier.journaltitlePLoS ONEen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/4132
dc.identifier.volume11
dc.language.isoenen
dc.publisherPLoSen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/613979/EU/Microbiome Influence on Energy balance and Brain Development-Function Put into Action to Tackle Diet-related Diseases and Behavior./MYNEWGUT
dc.relation.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0157527
dc.rights© 2016, Mazzola et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBifidobacteriumen
dc.subjectInfantsen
dc.subjectMicrobiomeen
dc.subjectAntibioticsen
dc.subjectEnterobacteriaceaeen
dc.subjectShannon indexen
dc.subjectBacteriaen
dc.subjectSimpson indexen
dc.titleEarly gut microbiota perturbations following intrapartum antibiotic prophylaxis to prevent group B streptococcal diseaseen
dc.typeArticle (peer-reviewed)en
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