dc.contributor.author	Batista-García, Ramón Alberto
dc.contributor.author	Sutton, Thomas
dc.contributor.author	Jackson, Stephen A.
dc.contributor.author	Tovar-Herrera, Omar Eduardo
dc.contributor.author	Balcázar-López, Edgar
dc.contributor.author	Sánchez-Carbente, María del Rayo
dc.contributor.author	Sánchez-Reyes, Ayixon
dc.contributor.author	Dobson, Alan D. W.
dc.contributor.author	Folch-Mallol, Jorge Luis
dc.contributor.funder	Seventh Framework Programme	en
dc.contributor.funder	Marine Institute	en
dc.contributor.funder	Consejo Nacional de Ciencia y Tecnología	en
dc.contributor.funder	European Molecular Biology Organization	en
dc.contributor.funder	Department of Agriculture, Food and the Marine	en
dc.date.accessioned	2017-04-28T11:59:45Z
dc.date.available	2017-04-28T11:59:45Z
dc.date.issued	2017-03
dc.date.updated	2017-04-28T11:42:36Z
dc.description.abstract	Extreme habitats have usually been regarded as a source of microorganisms that possess robust proteins that help enable them to survive in such harsh conditions. The deep sea can be considered an extreme habitat due to low temperatures (<5°C) and high pressure, however marine sponges survive in these habitats. While bacteria derived from deep-sea marine sponges have been studied, much less information is available on fungal biodiversity associated with these sponges. Following screening of fourteen fungi isolated from the deep-sea sponge Stelletta normani sampled at a depth of 751 metres, three halotolerant strains (TS2, TS11 and TS12) were identified which displayed high CMCase and xylanase activities. Molecular based taxonomic approaches identified these strains as Cadophora sp. TS2, Emericellopsis sp. TS11 and Pseudogymnoascus sp. TS 12. These three fungi displayed psychrotolerance and halotolerant growth on CMC and xylan as sole carbon sources, with optimal growth rates at 20°C. They produced CMCase and xylanase activities, which displayed optimal temperature and pH values of between 50–70°C and pH 5–8 respectively, together with good thermostability and halotolerance. In solid-state fermentations TS2, TS11 and TS12 produced CMCases, xylanases and peroxidase/phenol oxidases when grown on corn stover and wheat straw. This is the first time that CMCase, xylanase and peroxidase/phenol oxidase activities have been reported in these three fungal genera isolated from a marine sponge. Given the biochemical characteristics of these ligninolytic enzymes it is likely that they may prove useful in future biomass conversion strategies involving lignocellulosic materials.	en
dc.description.sponsorship	Marine Institute (Beaufort Marine Research Award, part of the Sea Change Strategy and the Strategy for Science Technology and Innovation (2006±2012), with the support of The Marine Institute under the Marine Research Sub-Programme of the National Development Plan 2007±2013); National Council for Science and Technology (CONACyT)-Mexico (Project CB-153789; and PRODEP-SEP Project UAEMOR-PTC-333) Europe Molecular Biology Organization (EMBO: ASTF503-2013); Department of Agriculture, Food and the Marine (DAFF11/F/009 MabS);	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	e0173750
dc.identifier.citation	Batista-García, R. A., Sutton, T., Jackson, S. A., Tovar-Herrera, O. E., Balcázar-López, E., Sánchez-Carbente, M. d. R., Sánchez-Reyes, A., Dobson, A. D. W. and Folch-Mallol, J. L. (2017) 'Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani', PLOS ONE, 12(3), pp. e0173750. doi:10.1371/journal.pone.0173750	en
dc.identifier.doi	10.1371/journal.pone.0173750
dc.identifier.endpage	e0173750-30	en
dc.identifier.issn	1932-6203
dc.identifier.issued	3	en
dc.identifier.journaltitle	Plos One	en
dc.identifier.startpage	e0173750-1	en
dc.identifier.uri	https://hdl.handle.net/10468/3903
dc.identifier.volume	12	en
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.relation.project	info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA	en
dc.relation.uri	http://dx.doi.org/10.1371%2Fjournal.pone.0173750
dc.rights	© 2017 Batista-García et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	en
dc.subject	Fungi	en
dc.subject	Sponges	en
dc.subject	Cellulases	en
dc.subject	Phylogenetics	en
dc.subject	Straw	en
dc.subject	Wheat	en
dc.subject	Maize	en
dc.subject	Phylogenetic analysis	en
dc.title	Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani	en
dc.type	Article (peer-reviewed)	en

Characterization of lignocellulolytic activities from fungi isolated from the deep-sea sponge Stelletta normani

Files

Original bundle

License bundle

Collections