Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments

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dc.contributor.authorRodriguez-Lopez, Pedro
dc.contributor.authorCarballo-Justo, Alba
dc.contributor.authorDraper, Lorraine A.
dc.contributor.authorCabo, Marta L.
dc.contributor.funderMinisterio de Economía y Competitividad
dc.contributor.funderEuropean Commission
dc.date.accessioned2018-07-30T10:30:33Z
dc.date.available2018-07-30T10:30:33Z
dc.date.issued2017
dc.description.abstractThe effects of pronase (PRN), cellulase (CEL) or DNaseI alone or combined with benzalkonium chloride (BAC) against Listeria monocytogenes-carrying biofilms were assayed. The best removal activity against L. monocytogenes-Escherichia coli biofilms was obtained using DNaseI followed by PRN and CEL. Subsequently, a modified logistic model was used to quantify the combined effects of PRN or DNaseI with BAC. A better BAC performance after PRN compared to DNaseI eradicating L. monocytogenes was observed. In E. coli the effects were the opposite. Finally, effects of DNaseI and DNaseI-BAC treatments were compared against two different L. monocytogenes-carrying biofilms. DNaseI-BAC was more effective against L. monocytogenes when co-cultured with E. coli. Nonetheless, comparing the removal effects after BAC addition, these were higher in mixed-biofilms with Pseudomonas fluorescens. However, a high number of released viable cells was observed after combined treatments. These results open new perspectives of enzymes as an anti-biofilm strategy for environmental pathogen control.en
dc.description.sponsorshipSpanish Ministerio de Economia y Competitividad (ENZYMONO) (AGL2010-22212-C02-02); FPI programme (BES-2011-050544)en
dc.description.sponsorshipMinisterio de Economía y Competitividad (ENZYMONO, AGL2010-22212-C02-02). European Commission (FPI programme Grant number: BES-2011- 050544)
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1080/08927014.2016.1261847
dc.identifier.endpage58
dc.identifier.issn0892-7014
dc.identifier.issued1
dc.identifier.journaltitleBiofoulingen
dc.identifier.startpage45
dc.identifier.urihttps://hdl.handle.net/10468/6525
dc.identifier.volume33
dc.language.isoenen
dc.publisherTaylor & Francis Ltden
dc.rights© 2016, Taylor & Francis Group. This is an Accepted Manuscript of an article published by Taylor & Francis in Biofouling on 5th December, 2016, available online: https://www.tandfonline.com/10.1080/08927014.2016.1261847. It is deposited under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectBenzalkonium chlorideen
dc.subjectBiofilmen
dc.subjectDisinfectionen
dc.subjectEnzymesen
dc.subjectFluorescence microscopyen
dc.subjectListeria monocytogenesen
dc.titleRemoval of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatmentsen
dc.typeArticle (peer-reviewed)en
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