The impact of complexation or complex coacervation of lactoferrin and osteopontin on simulated infant gastrointestinal digestion, intestinal inflammation, and in vivo bone development

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dc.check.date2025-09-09en
dc.check.infoAccess to this article is restricted until 12 months after publication by request of the publisheren
dc.contributor.authorGoulding, David A.en
dc.contributor.authorBonnet, Nicolasen
dc.contributor.authorHorcajada, Marie-Noëlleen
dc.contributor.authorBaruchet, Michaelen
dc.contributor.authorBermont, Flavienen
dc.contributor.authorHauser, Jonasen
dc.contributor.authorMacrì, Simoneen
dc.contributor.authorPisa, Edoardoen
dc.contributor.authorNembrini, Chiaraen
dc.contributor.authorVidal, Karineen
dc.contributor.authorO'Brien, Nora M.en
dc.contributor.authorO'Mahony, James A.en
dc.contributor.authorO'Regan, Jonathanen
dc.contributor.funderNestléen
dc.date.accessioned2024-10-03T14:00:47Z
dc.date.available2024-10-03T14:00:47Z
dc.date.issued2024-09-09en
dc.description.abstractLactoferrin (LF) and osteopontin (OPN) are bioactive milk proteins which can form heteroprotein complexes and complex coacervates. This research studied the effect of LF–OPN complexation and complex coacervation on the simulated infant gastrointestinal digestion of LF with subsequent examination of gut and bone health bioactivities in preclinical models. In an infant digestion model, the proteolytic profile of LF was unaltered by the pre-association of LF and OPN. Gastric proteolysis of LF was increased when the model gastric pH was reduced from 5.3 to 4.0, but less so when complexed with OPN. In a model of intestinal inflammation, undigested (79% inhibition) and gastric digestates (26% inhibition) of LF, but not gastrointestinal digestates, inhibited lipopolysaccharide (LPS)-induced NF-κB activation in intestinal epithelial cells. LF–OPN complexation sustained the inhibitory effect (21–43% of the undigested effect, depending on the type of complex) of LF after gastrointestinal digestion, suggesting that the peptides produced were different. In a neonatal rodent model used to study bone development, coacervating LF and OPN improved bone structures with a significant increase of trabecular proportion (BV/TV increase by 21.7%). This resulted in an 11.3% increase in stiffness of bones. Feeding the LF and OPN proteins in coacervate format also increased the levels of OPN, P1NP and M-CSF in blood, signifying a more pronounced impact on bone development. This research demonstrated that LF–OPN complexation and complex coacervation can delay simulated infant gastrointestinal digestion of LF and protect or improve the bioactivity of the proteins.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGoulding, D., Bonnet, N., Horcajada, M. N., Baruchet, M., Bermont, F., Hauser, J., Macrì, S., Pisa, E., Nembrini, C., Vidal, K. and O'Brien, N. (2024) 'The impact of complexation or complex coacervation of lactoferrin and osteopontin on simulated infant gastrointestinal digestion, intestinal inflammation, and in vivo bone development', Food & Function, 15(19), pp. 9928-9940. https://doi.org/10.1039/D4FO02790Fen
dc.identifier.doihttps://doi.org/10.1039/D4FO02790Fen
dc.identifier.eissn2042-650Xen
dc.identifier.endpage9940en
dc.identifier.issn2042-6496en
dc.identifier.issued19en
dc.identifier.journaltitleFood & Functionen
dc.identifier.startpage9928en
dc.identifier.urihttps://hdl.handle.net/10468/16502
dc.identifier.volume15en
dc.language.isoenen
dc.publisherRoyal Society of Chemistryen
dc.rights© 2024, Royal Society of Chemistry. This is the Accepted Manuscript version of a published work that appeared in final form in Food & Function, 15(19), pp. 9928-9940. The published version is available at: https://doi.org/10.1039/D4FO02790Fen
dc.subjectLactoferrinen
dc.subjectOsteopontinen
dc.subjectComplex coacervateen
dc.subjectDigestionen
dc.subjectBoneen
dc.titleThe impact of complexation or complex coacervation of lactoferrin and osteopontin on simulated infant gastrointestinal digestion, intestinal inflammation, and in vivo bone developmenten
dc.typeArticle (peer-reviewed)en
oaire.citation.issue19en
oaire.citation.volume15en
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