ΦCrAss001 represents the most abundant bacteriophage family in the human gut and infects Bacteroides intestinalis

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dc.contributor.authorShkoporov, Andrey N.
dc.contributor.authorKhokhlova, Ekaterina V.
dc.contributor.authorFitzgerald, C. Brian
dc.contributor.authorStockdale, Stephen R.
dc.contributor.authorDraper, Lorraine A.
dc.contributor.authorRoss, R. Paul
dc.contributor.authorHill, Colin
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Regional Development Funden
dc.contributor.funderJanssen Biotechen
dc.date.accessioned2019-07-18T12:20:29Z
dc.date.available2019-07-18T12:20:29Z
dc.date.issued2018-11-14
dc.description.abstractCrAssphages are an extensive and ubiquitous family of tailed bacteriophages, predicted to infect bacteria of the order Bacteroidales. Despite being found in ~50% of individuals and representing up to 90% of human gut viromes, members of this viral family have never been isolated in culture and remain understudied. Here, we report the isolation of a CrAssphage (ΦCrAss001) from human faecal material. This bacteriophage infects the human gut symbiont Bacteroides intestinalis, confirming previous in silico predictions of the likely host. DNA sequencing demonstrates that the bacteriophage genome is circular, 102 kb in size, and has unusual structural traits. In addition, electron microscopy confirms that ΦcrAss001 has a podovirus-like morphology. Despite the absence of obvious lysogeny genes, ΦcrAss001 replicates in a way that does not disrupt proliferation of the host bacterium, and is able to maintain itself in continuous host culture during several weeks.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid4781en
dc.identifier.citationShkoporov, A.N., Khokhlova, E.V., Fitzgerald, C.B., Stockdale, S.R., Draper, L.A., Ross, R.P. and Hill, C., 2018. ΦCrAss001 represents the most abundant bacteriophage family in the human gut and infects Bacteroides intestinalis. Nature communications, 9 (1): 4781. DOI:10.1038/s41467-018-07225-7en
dc.identifier.doi10.1038/s41467-018-07225-7en
dc.identifier.eissn2041-1723
dc.identifier.endpage8en
dc.identifier.issued1en
dc.identifier.journaltitleNature Communicationsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8204
dc.identifier.volume9en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Spokes Programme/14/SP APC/B3032/IE/Gut Phageomics - Phage as diagnostics and/or therapeutics in IBD/en
dc.relation.urihttps://www.nature.com/articles/s41467-018-07225-7
dc.rights© The Author(s) 2018en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectCrAssphagesen
dc.subjectBacteroidalesen
dc.subjectHuman gut viromesen
dc.subjectHost cultureen
dc.titleΦCrAss001 represents the most abundant bacteriophage family in the human gut and infects Bacteroides intestinalisen
dc.typeArticle (peer-reviewed)en
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