Evaluation of the cytotoxic effects of the novel antineoplastic agent 1,4,5-Oxathiazinane-4,4-dioxide on triple negative breast cancer cells

Simple item page
dc.contributor.authorJinih, Marcel
dc.contributor.authorWang, Jiang Huai
dc.contributor.authorPfirrmann, Rolf W.
dc.contributor.authorO'Leary, D. Peter
dc.contributor.authorCorrigan, Mark A.
dc.contributor.authorRedmond, Henry Paul
dc.date.accessioned2022-02-15T17:24:48Z
dc.date.available2022-02-15T17:24:48Z
dc.date.issued2021-05
dc.date.updated2022-02-15T17:16:06Z
dc.description.abstractBackground/Aim: Adjuvant therapeutic options are limited for triple negative breast cancer (TNBC). Thus, we evaluated the cytotoxic effects of the newly synthesized antineoplastic agent 1,4,5-Oxathiazinane-4,4-dioxide (OTD) on TNBC cells as a potential cancer therapeutic strategy. Materials and Methods: TNBC primary BT-20 and metastatic MDA-MB-231 cell lines were treated with increasing concentrations of OTD for various time periods to assess cell viability. Cell necrosis, apoptosis, necroptosis, autophagy, and ROS generation were evaluated using assay kits or specific inhibitors. Results: Treatment with OTD resulted in a dose-and time-dependent cell death of TNBC BT-20 and MDA-MB-231 cells. OTD also dose-dependently arrested TNBC cell proliferation. Notably, treatment with OTD induced both necrosis and apoptosis of TNBC cells, while the pan-caspase inhibitor Z-VAD-FMK partially attenuated OTD-induced cell death. Importantly, abrogated OTD-induced cell death was observed in the presence of the ROS scavenger N-acetylcysteine (NAC), whereas enhanced OTD-induced cell death was observed after the addition of the glutathione synthesis inhibitor BSO, indicating OTDinduced killing of TNBC cells via a reactive oxygen species dependent mechanism. Conclusion: OTD is strongly cytotoxic to both primary and metastatic TNBC cells, possibly by inducing multiple cell death pathways.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationJinih, M., Wang, J. H., Pfirrmann, R. W., O'Leary, D. P., Corrigan, M. A. and Redmond, H. P.(2021) 'Evaluation of the cytotoxic effects of the novel antineoplastic agent 1,4,5-Oxathiazinane-4,4-dioxide on triple negative breast cancer cells', Anticancer Research, 41(5), pp. 2247-2256. doi: 10.21873/anticanres.15001en
dc.identifier.doi10.21873/anticanres.15001en
dc.identifier.eissn1791-7530
dc.identifier.endpage2256en
dc.identifier.issn0250-7005
dc.identifier.issued5en
dc.identifier.journaltitleAnticancer Researchen
dc.identifier.startpage2247en
dc.identifier.urihttps://hdl.handle.net/10468/12570
dc.identifier.volume41en
dc.language.isoenen
dc.publisherInternational Institute of Anticancer Researchen
dc.rightsCopyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.en
dc.subjectOTDen
dc.subjectTriple-negative breast cancer cellsen
dc.subjectCytotoxicityen
dc.subjectCell deathen
dc.subjectReactive oxygen speciesen
dc.titleEvaluation of the cytotoxic effects of the novel antineoplastic agent 1,4,5-Oxathiazinane-4,4-dioxide on triple negative breast cancer cellsen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2247.full.pdf
Size:
956.96 KB
Format:
Adobe Portable Document Format
Description:
Published Version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Surgery - Journal Articles