Bioactivity screening and genomic analysis reveals deep-sea fish microbiome isolates as sources of novel antimicrobials

Simple item page
dc.contributor.authorUniacke-Lowe, Shonaen
dc.contributor.authorCollins, Fergus W. J.en
dc.contributor.authorHill, Colinen
dc.contributor.authorRoss, R. Paulen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2024-03-13T12:09:22Z
dc.date.available2024-03-13T12:09:22Z
dc.date.issued2023en
dc.description.abstractWith the increase in antimicrobial resistance and the subsequent demand for novel therapeutics, the deep-sea fish microbiome can be a relatively untapped source of antimicrobials, including bacteriocins. Previously, bacterial isolates were recovered from the gut of deep-sea fish sampled from the Atlantic Ocean.In this study, we used in vitro methods to screen a subset of these isolates for antimicrobial activity, and subsequently mined genomic DNA from isolates of interest for bacteriocin and other antimicrobial metabolite genes. We observed antimicrobial activity against foodborne pathogens, including Staphylococcus aureus, Listeria monocytogenes, Enterococcus faecalis and Micrococcus luteus. In total, 147 candidate biosynthetic gene clusters were identified in the genomic sequences, including 35 bacteriocin/RiPP-like clusters. Other bioactive metabolite genes detected included non-ribosomal peptide synthases (NRPS), polyketide synthases (PKS; Types 1 and 3), beta-lactones and terpenes. Moreover, four unique bacteriocin gene clusters were annotated and shown to encode novel peptides: a class IIc bacteriocin, two class IId bacteriocins and a class I lanthipeptide (LanM subgroup). Our dual in vitro and in silico approach allowed for a more comprehensive understanding of the bacteriocinogenic potential of these deep-sea isolates and an insight into the antimicrobial molecules that they may produce.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid444en
dc.identifier.citationUniacke-Lowe, S., Collins, F.W.J., Hill, C. and Ross, R.P. (2023) ‘Bioactivity screening and genomic analysis reveals deep-sea fish microbiome isolates as sources of novel antimicrobials’, Marine Drugs, 21(8), 444 (22pp). doi: 10.3390/md21080444en
dc.identifier.doi10.3390/md21080444en
dc.identifier.eissn1660-3397en
dc.identifier.endpage22en
dc.identifier.issued8en
dc.identifier.journaltitleMarine Drugsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15668
dc.identifier.volume21en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectAntimicrobialen
dc.subjectAntimicrobial resistanceen
dc.subjectBacteriocinsen
dc.subjectBiosynthetic gene clustersen
dc.subjectDeep-seaen
dc.subjectFish microbiomeen
dc.subjectGenome miningen
dc.titleBioactivity screening and genomic analysis reveals deep-sea fish microbiome isolates as sources of novel antimicrobialsen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
marinedrugs-21-00444-v2.pdf
Size:
5.11 MB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
marinedrugs-21-00444-s001.zip
Size:
14.18 MB
Format:
http://www.iana.org/assignments/media-types/application/zip
Description:
Supplementary Materials
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles