dc.contributor.author	Hill, Cian J.
dc.contributor.author	Lynch, Denise B.
dc.contributor.author	Murphy, Kiera
dc.contributor.author	Ulaszewska, Marynka
dc.contributor.author	Jeffery, Ian B.
dc.contributor.author	O'Shea, Carol Anne
dc.contributor.author	Watkins, Claire
dc.contributor.author	Dempsey, Eugene M.
dc.contributor.author	Mattivi, Fulvio
dc.contributor.author	Tuohy, Kieran
dc.contributor.author	Ross, R. Paul
dc.contributor.author	Ryan, C. Anthony
dc.contributor.author	O'Toole, Paul W.
dc.contributor.author	Stanton, Catherine
dc.contributor.funder	Department of Agriculture, Food and the Marine	en
dc.contributor.funder	Health Research Board	en
dc.date.accessioned	2017-02-27T18:41:53Z
dc.date.available	2017-02-27T18:41:53Z
dc.date.issued	2017-01-17
dc.date.updated	2017-02-27T10:29:38Z
dc.description.abstract	Background: The gut is the most extensively studied niche of the human microbiome. The aim of this study was to characterise the initial gut microbiota development of a cohort of breastfed infants (n = 192) from 1 to 24 weeks of age. Methods: V4-V5 region 16S rRNA amplicon Illumina sequencing and, in parallel, bacteriological culture. The metabolomic profile of infant urine at 4 weeks of age was also examined by LC-MS. Results: Full-term (FT), spontaneous vaginally delivered (SVD) infants’ microbiota remained stable at both phylum and genus levels during the 24-week period examined. FT Caesarean section (CS) infants displayed an increased faecal abundance of Firmicutes (p < 0.01) and lower abundance of Actinobacteria (p < 0.001) after the first week of life compared to FT-SVD infants. FT-CS infants gradually progressed to harbouring a microbiota closely resembling FT-SVD (which remained stable) by week 8 of life, which was maintained at week 24. The gut microbiota of preterm (PT) infants displayed a significantly greater abundance of Proteobacteria compared to FT infants (p < 0.001) at week 1. Metabolomic analysis of urine at week 4 indicated PT-CS infants have a functionally different metabolite profile than FT (both CS and SVD) infants. Co-inertia analysis showed co-variation between the urine metabolome and the faecal microbiota of the infants. Tryptophan and tyrosine metabolic pathways, as well as fatty acid and bile acid metabolism, were found to be affected by delivery mode and gestational age. Conclusions: These findings confirm that mode of delivery and gestational age both have significant effects on early neonatal microbiota composition. There is also a significant difference between the metabolite profile of FT and PT infants. Prolonged breastfeeding was shown to have a significant effect on the microbiota composition of FT-CS infants at 24 weeks of age, but interestingly not on that of FT-SVD infants. Twins had more similar microbiota to one another than between two random infants, reflecting the influence of similarities in both host genetics and the environment on the microbiota.	en
dc.description.sponsorship	Department of Agriculture, Food and Marine (FIRM grant to the INFANTMET project); Health Research Board (Grant Number: HRA_POR/2012/123)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Hill, C. J., Lynch, D. B., Murphy, K., Ulaszewska, M., Jeffery, I. B., O’Shea, C. A., Watkins, C., Dempsey, E., Mattivi, F., Tuohy, K., Ross, R. P., Ryan, C. A., O’ Toole, P. W. and Stanton, C. (2017) ‘Evolution of gut microbiota composition from birth to 24 weeks in the INFANTMET Cohort’, Microbiome, 5(4), pp. 1-18. doi:10.1186/s40168-016-0213-y	en
dc.identifier.doi	10.1186/s40168-016-0213-y
dc.identifier.endpage	18	en
dc.identifier.issn	2049-2618
dc.identifier.issued	4	en
dc.identifier.journaltitle	Microbiome	en
dc.identifier.startpage	1	en
dc.identifier.uri	https://hdl.handle.net/10468/3701
dc.identifier.volume	5	en
dc.language.iso	en	en
dc.publisher	BioMed Central	en
dc.rights	© 2017, the Authors. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	en
dc.subject	Metabolite profile	en
dc.subject	Breastfeeding	en
dc.subject	Microbiota	en
dc.title	Evolution of gut microbiota composition from birth to 24 weeks in the INFANTMET Cohort	en
dc.type	Article (peer-reviewed)	en

Evolution of gut microbiota composition from birth to 24 weeks in the INFANTMET Cohort

Files

Original bundle

License bundle

Collections