Inhibitory activity of Lactobacillus plantarum LMG P-26358 against Listeria innocua when used as an adjunct starter in the manufacture of cheese

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dc.contributor.authorMills, S.
dc.contributor.authorSerrano, L. M.
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorGriffin, Carmel
dc.contributor.authorSchaad, Gwenda
dc.contributor.authorBruining, Chris
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.contributor.authorMeijer, Wilco C.
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2013-04-08T15:46:14Z
dc.date.available2013-04-08T15:46:14Z
dc.date.copyright2011-08
dc.date.issued2011-08
dc.description.abstractLactobacillus plantarum LMG P-26358 isolated from a soft French artisanal cheese produces a potent class IIa bacteriocin with 100% homology to plantaricin 423 and bacteriocidal activity against Listeria innocua and Listeria monocytogenes. The bacteriocin was found to be highly stable at temperatures as high as 100°C and pH ranges from 1-10. While this relatively narrow spectrum bacteriocin also exhibited antimicrobial activity against species of enterococci, it did not inhibit dairy starters including lactococci and lactobacilli when tested by well diffusion assay (WDA). In order to test the suitability of Lb. plantarum LMG P-26358 as an anti-listerial adjunct with nisin-producing lactococci, laboratory-scale cheeses were manufactured. Results indicated that combining Lb. plantarum LMG P-26358 (at 108 colony forming units (cfu)/ml) with a nisin producer is an effective strategy to eliminate the biological indicator strain, L. innocua. Moreover, industrial-scale cheeses also demonstrated that Lb. plantarum LMG P-26358 was much more effective than the nisin producer alone for protection against the indicator. MALDI-TOF mass spectrometry confirmed the presence of plantaricin 423 and nisin in the appropriate cheeses over an 18 week ripening period. A spray-dried fermentate of Lb. plantarum LMG P-26358 also demonstrated potent anti-listerial activity in vitro using L. innocua. Overall, the results suggest that Lb. plantarum LMG P-26358 is a suitable adjunct for use with nisin-producing cultures to improve the safety and quality of dairy productsen
dc.description.sponsorshipScience Foundation Ireland (SFI-CSET);en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMills, S., Serrano, L.M., Griffin, C., O. Connor, P.M, Schaad G., Bruining C., Hill, C., Ross, R.P., Meijer, W.C. (2011) Inhibitory Activity of Lactobacillus plantarum LMG P-26358 against Listeria innocua when used as an Adjunct Starter in the Manufacture of Cheese. Microbial Cell Factories 10(Suppl 1):S7en
dc.identifier.doi10.1186/1475-2859-10-S1-S7
dc.identifier.issued(Suppl 1):S7en
dc.identifier.journaltitleMicrobial Cell Factoriesen
dc.identifier.urihttps://hdl.handle.net/10468/1032
dc.identifier.volume10en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urihttp://www.microbialcellfactories.com/content/10/S1/S7
dc.rights© 2011 Mills et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en
dc.subjectLactobacillus plantarumen
dc.subjectFrench artisanal cheeseen
dc.subjectBacteriocinen
dc.subjectPlantaricin 423en
dc.subjectListeria innocuaen
dc.subjectListeria monocytogenesen
dc.subjectNisin-producing cultureen
dc.subject.lcshMicrobiology.en
dc.titleInhibitory activity of Lactobacillus plantarum LMG P-26358 against Listeria innocua when used as an adjunct starter in the manufacture of cheeseen
dc.typeArticle (peer-reviewed)en
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