Strain-level variation among vaginal Lactobacillus crispatus and Lactobacillus iners as identified by comparative metagenomics

Simple item page
dc.contributor.authorNori, Sai Ravi Chandraen
dc.contributor.authorWalsh, Calum J.en
dc.contributor.authorMcAuliffe, Fionnuala M.en
dc.contributor.authorMoore, Rebecca L.en
dc.contributor.authorVan Sinderen, Douween
dc.contributor.authorFeehily, Conoren
dc.contributor.authorCotter, Paul D.en
dc.contributor.funderHorizon 2020en
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2025-04-15T11:44:03Z
dc.date.available2025-04-15T11:44:03Z
dc.date.issued2025en
dc.description.abstractThe vaginal microbiome, a relatively simple, low diversity ecosystem crucial for female health, is often dominated by Lactobacillus spp. Detailed strain-level data, facilitated by shotgun sequencing, can provide a greater understanding of the mechanisms of colonization and host-microbe interactions. We analysed 354 vaginal metagenomes from pregnant women in Ireland to investigate metagenomic community state types and strain-level variation, focusing on cell surface interfaces. Our analysis revealed multiple subspecies, with Lactobacillus crispatus and Lactobacillus iners being the most dominant. We found genes, including putative mucin-binding genes, distinct to L. crispatus subspecies. Using 337 metagenome-assembled genomes, we observed a higher number of strain-specific genes in L. crispatus related to cell wall biogenesis, carbohydrate and amino acid metabolism, many under positive selection. A cell surface glycan gene cluster was predominantly found in L. crispatus but absent in L. iners and Gardnerella vaginalis. These findings highlight strain-specific factors associated with colonisation and host-microbe interactions. © The Author(s) 2025.en
dc.description.sponsorshipScience Foundation Ireland (18/CRT/6214; SFI/12/RC/2273; 16/SP/3827); Horizon 2020 (H2020-MSCA-COFUND-2019-945385) en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid49en
dc.identifier.citationNori, S. R. C., Walsh, C. J., McAuliffe, F. M., Moore, R. L., Van Sinderen, Douwe, Feehily, C. and Cotter, Paul D. (2025) 'Strain-level variation among vaginal Lactobacillus crispatus and Lactobacillus iners as identified by comparative metagenomics', npj Biofilms and Microbiomes, 11(1), p.49. https://doi.org/10.1038/s41522-025-00682-1en
dc.identifier.doi10.1038/s41522-025-00682-1en
dc.identifier.issn20555008en
dc.identifier.issued1
dc.identifier.journaltitlenpj Biofilms and Microbiomesen
dc.identifier.urihttps://hdl.handle.net/10468/17268
dc.identifier.volume11
dc.language.isoenen
dc.publisherNature Researchen
dc.rights© 2025, the Author(s). Open Access.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAmino aciden
dc.subjectCarbohydrateen
dc.subjectGlycanen
dc.subjectMucinen
dc.subjectAccessory genomeen
dc.titleStrain-level variation among vaginal Lactobacillus crispatus and Lactobacillus iners as identified by comparative metagenomicsen
dc.typeArticle (peer reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s41522-025-00682-1.pdf
Size:
3 MB
Format:
Adobe Portable Document Format
Description:
Published Version
Download
Collections
APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles