A bioengineered nisin derivative to control biofilms of Staphylococcus pseudintermedius

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dc.contributor.authorField, Des
dc.contributor.authorGaudin, Noemie
dc.contributor.authorLyons, Francy
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorCotter, Paul D.
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderIrish Government
dc.contributor.funderSociety for Applied Microbiology, United Kingdom
dc.date.accessioned2016-02-17T10:07:59Z
dc.date.available2016-02-17T10:07:59Z
dc.date.issued2015
dc.description.abstractAntibiotic resistance and the shortage of novel antimicrobials are among the biggest challenges facing society. One of the major factors contributing to resistance is the use of frontline clinical antibiotics in veterinary practice. In order to properly manage dwindling antibiotic resources, we must identify antimicrobials that are specifically targeted to veterinary applications. Nisin is a member of the lantibiotic family of antimicrobial peptides that exhibit potent antibacterial activity against many gram-positive bacteria, including human and animal pathogens such as Staphylococcus, Bacillus, Listeria, and Clostridium. Although not currently used in human medicine, nisin is already employed commercially as an anti-mastitis product in the veterinary field. Recently we have used bioengineering strategies to enhance the activity of nisin against several high profile targets, including multi-drug resistant clinical pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) and also against staphylococci and streptococci associated with bovine mastitis. However, newly emerging pathogens such as methicillin resistant Staphylococcus pseudintermedius (MRSP) pose a significant threat in terms of veterinary health and as a reservoir for antibiotic resistance determinants. In this study we created a nisin derivative with enhanced antimicrobial activity against S. pseudintermedius. In addition, the novel nisin derivative exhibits an enhanced ability to impair biofilm formation and to reduce the density of established biofilms. The activities of this peptide represent a significant improvement over that of the wild-type nisin peptide and merit further investigation with a view to their use to treat S. pseudintermedius infections.en
dc.description.sponsorshipScience Foundation Ireland (Investigator Award 10/IN. 1/B3027); Society for Applied Microbiology, United Kingdom (Students into Work Grant)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0119684
dc.identifier.citationField D, Gaudin N, Lyons F, O'Connor PM, Cotter PD, Hill C, et al. (2015) A Bioengineered Nisin Derivative to Control Biofilms of Staphylococcus pseudintermedius. PLoS ONE 10(3): e0119684. doi:10.1371/journal.pone.0119684
dc.identifier.doi10.1371/journal.pone.0119684
dc.identifier.issn1932-6203
dc.identifier.issued3en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.urihttps://hdl.handle.net/10468/2314
dc.identifier.volume10en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2015 Field et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAntimicrobial cationic peptidesen
dc.subjectIn vitro activitiesen
dc.subjectMethicillin-resistanten
dc.subjectEnhanced activityen
dc.subjectAureus biofilmsen
dc.subjectLantibioticsen
dc.subjectCombinationen
dc.subjectCowsen
dc.subjectBiosynthesisen
dc.subjectBacteriocinsen
dc.titleA bioengineered nisin derivative to control biofilms of Staphylococcus pseudintermediusen
dc.typeArticle (peer-reviewed)en
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