Localisation and substrate specificities of transglycanases in charophyte algae relate to development and morphology

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dc.contributor.authorHerburger, Klaus
dc.contributor.authorRyan, Louise M.
dc.contributor.authorPopper, Zoe A.
dc.contributor.authorHolzinger, Andreas
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderMedizinische Universität Innsbruck
dc.contributor.funderAustrian Science Fund
dc.date.accessioned2018-06-15T11:47:09Z
dc.date.available2018-06-15T11:47:09Z
dc.date.issued2018
dc.description.abstractCell wall-modifying enzymes have been previously investigated in charophyte green algae (CGA) in cultures of uniform age, giving limited insight into their roles. Therefore, we investigated the in situ localisation and specificity of enzymes acting on hemicelluloses in CGA genera of different morphologies and developmental stages. In vivo transglycosylation between xyloglucan and an endogenous donor in filamentous Klebsormidium and Zygnema was observed in longitudinal cell walls of young (1 month) but not old cells (1 year), suggesting that it has a role in cell growth. By contrast, in parenchymatous Chara, transglycanase action occurred in all cell planes. In Klebsormidium and Zygnema, the location of enzyme action mainly occurred in regions where xyloglucans and mannans, and to a lesser extent mixed-linkage beta-glucan (MLG), were present, indicating predominantly xyloglucan: xyloglucan endotransglucosylase (XET) activity. Novel transglycosylation activities between xyloglucan and xylan, and xyloglucan and galactomannan were identified in vitro in both genera. Our results show that several cell wall-modifying enzymes are present in CGA, and that differences in morphology and cell age are related to enzyme localisation and specificity. This indicates an evolutionary significance of cell wall modifications, as similar changes are known in their immediate descendants, the land plants. This article has an associated First Person interview with the first author of the paper.en
dc.description.sponsorshipAustrian Science Fund (P 24242-B16, I 1951-B16)en
dc.description.statusPeer reviewed
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleidUNSP jcs203208
dc.identifier.citationHerburger, K., Ryan, L. M., Popper, Z. A. and Holzinger, A. (2018) 'Localisation and substrate specificities of transglycanases in charophyte algae relate to development and morphology', Journal of Cell Science, 131, jcs203208 (10pp). doi: 10.1242/jcs.203208en
dc.identifier.doi10.1242/jcs.203208
dc.identifier.endpage10
dc.identifier.issn0021-9533
dc.identifier.issued2
dc.identifier.journaltitleJournal of Cell Scienceen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/6320
dc.identifier.volume131
dc.language.isoenen
dc.publisherCompany of Biologistsen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Frontiers Programme (RFP)/11/RFP.1/EOB/3345/IE/Characterisation of algal cell wall components for future biotechnological applications/
dc.relation.urihttp://jcs.biologists.org/content/131/2/jcs203208
dc.rights© 2018, Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0
dc.subjectCharophyte green algaeen
dc.subjectMixed-linkage beta-glucanen
dc.subjectTransglycosylationen
dc.subjectXyloglucan transglucosylase hydrolaseen
dc.subjectXylanen
dc.subjectXyloglucanen
dc.titleLocalisation and substrate specificities of transglycanases in charophyte algae relate to development and morphologyen
dc.typeArticle (peer-reviewed)en
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