Kidney dopamine D1-like receptors and angiotensin 1-7 interaction inhibits renal Na+ transporters
dc.contributor.author | Banday, Anees A. | |
dc.contributor.author | Diaz, Andrea | |
dc.contributor.author | Lokhandwala, Mustafa | |
dc.contributor.funder | National Heart, Lung, and Blood Institute | en |
dc.date.accessioned | 2021-09-23T14:04:46Z | |
dc.date.available | 2021-09-23T14:04:46Z | |
dc.date.issued | 2019-10-09 | |
dc.date.updated | 2021-09-23T13:54:22Z | |
dc.description.abstract | The role of dopamine D1-like receptors (DR) in the regulation of renal Na+ transporters, natriuresis, and blood pressure is well established. However, the involvement of the angiotensin 1-7 (ANG 1-7)-Mas receptor in the regulation of Na+ balance and blood pressure is not clear. The present study aimed to investigate the hypothesis that ANG 1-7 can regulate Na+ homeostasis by modulating the renal dopamine system. Sprague-Dawley rats were infused with saline alone (vehicle) or saline with ANG 1-7, ANG 1-7 antagonist A-779, DR agonist SKF38393, and antagonist SCH23390. Infusion of ANG 1-7 caused significant natriuresis and diuresis compared with saline alone. Both natriuresis and diuresis were blocked by A-779 and SCH23390. SKF38393 caused a significant, SCH23390-sensitive natriuresis and diuresis, and A-779 had no effect on the SKF38393 response. Concomitant infusion of ANG 1-7 and SKF38393 did not show a cumulative effect compared with either agonist alone. Treatment of renal proximal tubules with ANG 1-7 or SKF38393 caused a significant decrease in Na+-K+-ATPase and Na+/H+ exchanger isoform 3 activity. While SCH23390 blocked both ANG 1-7- and SKF38393-induced inhibition, the DR response was not sensitive to A-779. Additionally, ANG 1-7 activated PKG, enhanced tyrosine hydroxylase activity via Ser40 phosphorylation, and increased renal dopamine production. These data suggest that ANG 1-7, via PKG, enhances tyrosine hydroxylase activity, which increases renal dopamine production and activation of DR and subsequent natriuresis. This study provides evidence for a unidirectional functional interaction between two G protein-coupled receptors to regulate renal Na+ transporters and induce natriuresis. | en |
dc.description.sponsorship | National Heart, Lung, and Blood Institute (Grant HL-139808) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Accepted Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.citation | Banday, A. A., Diaz, A. and Lokhandwala, M. (2019) 'Kidney dopamine D1-like receptors and angiotensin 1-7 interaction inhibits renal Na+ transporters', American Journal of Physiology - Renal Physiology, 317(4), pp. F949-F956. doi: 10.1152/ajprenal.00135.2019 | en |
dc.identifier.doi | 10.1152/ajprenal.00135.2019 | en |
dc.identifier.eissn | 1522-1466 | |
dc.identifier.endpage | F956 | en |
dc.identifier.issn | 1931-857X | |
dc.identifier.issued | 4 | en |
dc.identifier.journaltitle | American Journal of Physiology - Renal Physiology | en |
dc.identifier.startpage | F949 | en |
dc.identifier.uri | https://hdl.handle.net/10468/11994 | |
dc.identifier.volume | 317 | en |
dc.language.iso | en | en |
dc.publisher | American Physiological Society | en |
dc.rights | © 2019, American Physiological Society. All rights reserved. This is a post-peer-review, pre-copyedit version of an article published in American Journal of Physiology - Renal Physiology. The final authenticated version is available online at: https://doi.org/10.1152/ajprenal.00135.2019 | en |
dc.subject | Na-K-ATPase | en |
dc.subject | Na/H exchanger | en |
dc.subject | Natriuresis | en |
dc.subject | Renal tubules | en |
dc.title | Kidney dopamine D1-like receptors and angiotensin 1-7 interaction inhibits renal Na+ transporters | en |
dc.type | Article (peer-reviewed) | en |