Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain

dc.contributor.authorLiu, Wei
dc.contributor.authorXiao, Shaobo
dc.contributor.authorLi, Mao
dc.contributor.authorGuo, Shaohua
dc.contributor.authorLi, Sha
dc.contributor.authorLuo, Rui
dc.contributor.authorFeng, Zhixin
dc.contributor.authorLi, Bin
dc.contributor.authorZhou, Zhemin
dc.contributor.authorShao, Guoqing
dc.contributor.authorChen, Huanchun
dc.contributor.authorFang, Liurong
dc.contributor.funderNational Natural Science Foundation of China
dc.date.accessioned2016-02-25T17:16:20Z
dc.date.available2016-02-25T17:16:20Z
dc.date.issued2013-02-05
dc.description.abstractBackground: Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia (EP), a mild, chronic pneumonia of swine. Despite presenting with low direct mortality, EP is responsible for major economic losses in the pig industry. To identify the virulence-associated determinants of M. hyopneumoniae, we determined the whole genome sequence of M. hyopneumoniae strain 168 and its attenuated high-passage strain 168-L and carried out comparative genomic analyses. Results: We performed the first comprehensive analysis of M. hyopneumoniae strain 168 and its attenuated strain and made a preliminary survey of coding sequences (CDSs) that may be related to virulence. The 168-L genome has a highly similar gene content and order to that of 168, but is 4,483 bp smaller because there are 60 insertions and 43 deletions in 168-L. Besides these indels, 227 single nucleotide variations (SNVs) were identified. We further investigated the variants that affected CDSs, and compared them to reported virulence determinants. Notably, almost all of the reported virulence determinants are included in these variants affected CDSs. In addition to variations previously described in mycoplasma adhesins (P97, P102, P146, P159, P216, and LppT), cell envelope proteins (P95), cell surface antigens (P36), secreted proteins and chaperone protein (DnaK), mutations in genes related to metabolism and growth may also contribute to the attenuated virulence in 168-L. Furthermore, many mutations were located in the previously described repeat motif, which may be of primary importance for virulence. Conclusions: We studied the virulence attenuation mechanism of M. hyopneumoniae by comparative genomic analysis of virulent strain 168 and its attenuated high-passage strain 168-L. Our findings provide a preliminary survey of CDSs that may be related to virulence. While these include reported virulence-related genes, other novel virulence determinants were also detected. This new information will form the foundation of future investigations into the pathogenesis of M. hyopneumoniae and facilitate the design of new vaccines.en
dc.description.sponsorshipNational Natural Science Foundation of China (31170160, 31001080, and 31001066)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid80
dc.identifier.citationLIU, W., XIAO, S., LI, M., GUO, S., LI, S., LUO, R., FENG, Z., LI, B., ZHOU, Z., SHAO, G., CHEN, H. & FANG, L. 2013. Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain. BMC Genomics, 14:80, 1-13. http://dx.doi.org/10.1186/1471-2164-14-80en
dc.identifier.doi10.1186/1471-2164-14-80
dc.identifier.endpage13en
dc.identifier.issn1471-2164
dc.identifier.journaltitleBMC Genomicsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/2402
dc.identifier.volume14en
dc.language.isoenen
dc.publisherBioMed Central Ltd.en
dc.rights© Liu et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://​creativecommons.​org/​licenses/​by/​2.​0en
dc.subjectMycoplasma hyopneumoniaeen
dc.subjectGenetic variationen
dc.subjectVirulence attenuationen
dc.subjectSequence analysisen
dc.subjectRepetitive sequencesen
dc.subjectVirulence factorsen
dc.titleComparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strainen
dc.typeArticle (peer-reviewed)en
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