Interaction between nitric oxide and renal α1-adrenoreceptors mediated vasoconstriction in rats with left ventricular hypertrophyin Wistar Kyoto rats

Ahmad, Ashfaq; Sattar, Munavvar A.; Azam, Maleeha; Khan, Safia A.; Bhatt, Owais; Johns, Edward J.

Interaction between nitric oxide and renal α1-adrenoreceptors mediated vasoconstriction in rats with left ventricular hypertrophyin Wistar Kyoto rats

dc.contributor.author	Ahmad, Ashfaq
dc.contributor.author	Sattar, Munavvar A.
dc.contributor.author	Azam, Maleeha
dc.contributor.author	Khan, Safia A.
dc.contributor.author	Bhatt, Owais
dc.contributor.author	Johns, Edward J.
dc.contributor.funder	Institute of Postgraduate Studies, Universiti Sains Malaysia
dc.contributor.funder	Universiti Sains Malaysia
dc.date.accessioned	2018-05-02T10:16:23Z
dc.date.available	2018-05-02T10:16:23Z
dc.date.issued	2018
dc.description.abstract	Left ventricular hypertrophy (LVH) is associated with decreased responsiveness of renal alpha(1)-adrenoreceptors subtypes to adrenergic agonists. Nitric oxide donors are known to have antihypertrophic effects however their impact on responsiveness of renal alpha(1)-adrenoreceptors subtypes is unknown. This study investigated the impact of nitric oxide (NO) and its potential interaction with the responsiveness of renal alpha(1)-adrenoreceptors subtypes to adrenergic stimulation in rats with left ventricular hypertrophy (LVH). This study also explored the impact of NO donor on CSE expression in normal and LVH kidney. LVH was induced using isoprenaline and caffeine in drinking water for 2 weeks while NO donor (L-arginine, 1.25g/Lin drinking water) was given for 5 weeks. Intrarenal noradrenaline, phenylephrine and methoxamine responses were determined in the absence and presence of selective alpha(1)-adrenoceptor antagonists, 5- methylurapidil (5-MeU), chloroethylclonidine (CeC) and BMY 7378. Renal cortical endothelial nitric oxide synthase mRNA was upregulated 7 fold while that of cystathione Upsilon lyase was unaltered in the NO treated LVH rats (LVH-NO) group compared to LVH group. The responsiveness of renal alpha(1A), alpha(1B) and alpha(1D)-adrenoceptors in the low dose and high dose phases of 5-MeU, CEC and BMY7378 to adrenergic agonists was increased along with cGMP in the kidney of LVH-NO group. These findings suggest that exogenous NO precursor up-regulated the renal eNOS/NO/cGMP pathway in LVH rats and resulted in augmented alpha(1A), alpha(1B) and alpha(1D) adrenoreceptors responsiveness to the adrenergic agonists. There is a positive interaction between H2S and NO production in normal animals but this interaction appears absent in LVH animals.	en
dc.description.sponsorship	Institute of Postgraduate Studies, Universiti Sains Malaysia ( USM fellowship (APEX (1002/JHEA/ATSG4001); Universiti Sains Malaysia (1001/PFARMASI/815078)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	e0189386
dc.identifier.citation	Ahmad, A., Sattar, M. A., Azam, M., Khan, S. A., Bhatt, O. and Johns, E. J. (2018) 'Interaction between nitric oxide and renal α1-adrenoreceptors mediated vasoconstriction in rats with left ventricular hypertrophyin Wistar Kyoto rats', PLOS ONE, 13(2), e0189386 (21pp). doi: 10.1371/journal.pone.0189386	en
dc.identifier.doi	10.1371/journal.pone.0189386
dc.identifier.issn	1932-6203
dc.identifier.issued	2
dc.identifier.journaltitle	PLOS ONE	en
dc.identifier.uri	https://hdl.handle.net/10468/5937
dc.identifier.volume	13
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.relation.uri	http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189386
dc.rights	© 2018, Ahmad et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Kidneys	en
dc.subject	Blood plasma	en
dc.subject	Blood	en
dc.subject	Renal system	en
dc.subject	Collagens	en
dc.subject	Vasoconstriction	en
dc.subject	Cardiac hypertrophy	en
dc.title	Interaction between nitric oxide and renal α1-adrenoreceptors mediated vasoconstriction in rats with left ventricular hypertrophyin Wistar Kyoto rats	en
dc.type	Article (peer-reviewed)	en