Pen direct writing of multiplex-LFIA for detection of thiamphenicol and tylosin in milk

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dc.check.date2026-01-09en
dc.check.infoAccess to this article is restricted until 12 months after publication by request of the publisheren
dc.contributor.authorRusso, Alidaen
dc.contributor.authorCavalera, Simoneen
dc.contributor.authorMurray, Richarden
dc.contributor.authorQuinn, Aidan J.en
dc.contributor.authorAnfossi, Lauraen
dc.contributor.authorIacopino, Danielaen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Regional Development Funden
dc.date.accessioned2025-02-05T09:57:10Z
dc.date.available2025-02-05T09:57:10Z
dc.date.issued2025-01-09en
dc.description.abstractTherapeutic and misuse of veterinary drugs, such as antibiotics, can increase the potential risk of residue contamination in animal-derived food products. For milk, these residual antibiotics can have an impact on efficiency in dairy processing factories, as well as economic loss, and can also cause side effects on consumer health. Lateral flow immunoassays (LFIAs) are gaining popularity for their ease of use, low cost and their fulfilment to the REASSURED (real-time connection/monitoring, easy sampling, affordable, specific, user-friendly, rapid/robust, equipment free, deliverable to end user) criteria. At the same time, direct writing of functional materials has been recently used for facile applications on point-of-care test (POCT) fabrication. Herein, a novel multiplex-LFIA has been developed for the simultaneous detection of two antibiotics in milk: thiamphenicol and tylosin. In contrast to the traditional automated dispensers, a direct pen writing method was used to deposit control and test lines. The response of each antibiotic was tested, as well as the selectivity of the test during cross-testing. For both antibiotics, the test was successful in distinguishing between safe and unsafe milk samples, according to the maximum residue limits (MRLs) set for both antibiotics. The visual LOD obtained was 100 ppb for the two antibiotics, and the IC50 was lower than the set maximum residue limits (MRLs).en
dc.description.sponsorshipScience Foundation Ireland (13/RC/2077-P2 CONNECT); European Regional Development Fund (TECH4MILK Regione Piemonte, F.E.S.R. 2014/2020 e F.S.E. 2014/2020)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid59en
dc.identifier.citationRusso, A., Cavalera, S., Murray, R., Quinn, A. J., Anfossi, L. and Iacopino, D. (2025) 'Pen direct writing of multiplex-LFIA for detection of thiamphenicol and tylosin in milk', Microchimica Acta, 192, 59 (11pp). https://doi.org/10.1007/s00604-024-06900-5en
dc.identifier.doihttps://doi.org/10.1007/s00604-024-06900-5en
dc.identifier.eissn1436-5073en
dc.identifier.endpage11en
dc.identifier.issn0026-3672en
dc.identifier.journaltitleMicrochimica Actaen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/16965
dc.identifier.volume192en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.ispartofMicrochimica Actaen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres Programme::Phase 1/16/RC/3835/IE/VistaMilk Centre/en
dc.relation.project13/RC/2077-P2en
dc.rights© 2025, the Authors, under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. This version of the paper has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s00604-024-06900-5en
dc.subjectLFIAen
dc.subjectGold nanoparticlesen
dc.subjectMilk analysisen
dc.subjectAntibioticsen
dc.subjectDirect writingen
dc.subjectVisual detectionen
dc.subjectSmartphone RGB color detectionen
dc.subjectImage Jen
dc.titlePen direct writing of multiplex-LFIA for detection of thiamphenicol and tylosin in milken
dc.typeArticle (peer-reviewed)en
oaire.citation.issue2en
oaire.citation.volume192en
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