Perstraction of intracellular pigments through submerged fermentation of Talaromyces spp. in a surfactant rich media: a novel approach for enhanced pigment recovery

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dc.contributor.authorMorales-Oyervides, Lourdes
dc.contributor.authorOliveira, Jorge C.
dc.contributor.authorSousa-Gallagher, Maria J.
dc.contributor.authorMéndez-Zavala, Alejandro
dc.contributor.authorMontañez, Julio
dc.contributor.funderConsejo Nacional de Ciencia y Tecnología
dc.date.accessioned2017-10-18T09:40:11Z
dc.date.available2017-10-18T09:40:11Z
dc.date.issued2017
dc.description.abstractA high percentage of the pigments produced by Talaromyces spp. remains inside the cell, which could lead to a high product concentration inhibition. To overcome this issue an extractive fermentation process, perstraction, was suggested, which involves the extraction of the intracellular products out of the cell by using a two-phase system during the fermentation. The present work studied the effect of various surfactants on secretion of intracellular pigments produced by Talaromyces spp. in submerged fermentation. Surfactants used were: non-ionic surfactants (Tween 80, Span 20 and Triton X-100) and a polyethylene glycerol polymer 8000, at different concentrations (5, 20, 35 g/L). The highest extracellular pigment yield (16 OD500nm) was reached using Triton X-100 (35 g/L), which was 44% higher than the control (no surfactant added). The effect of addition time of the selected surfactant was further studied. The highest extracellular pigment concentration (22 OD500nm) was achieved when the surfactant was added at 120 h of fermentation. Kinetics of extracellular and intracellular pigments were examined. Total pigment at the end of the fermentation using Triton X-100 was 27.7% higher than the control, confirming that the use of surfactants partially alleviated the product inhibition during the pigment production cultureen
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología (215490-2011)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid33
dc.identifier.citationMorales-Oyervides, L., Oliveira, J., Sousa-Gallagher, M., Méndez-Zavala, A. and Montañez, J. (2017) 'Perstraction of intracellular pigments through submerged fermentation of Talaromyces spp. in a surfactant rich media: a novel approach for enhanced pigment recovery', Journal of Fungi, 3(3), 33 (12pp). doi: 10.3390/jof3030033en
dc.identifier.doi10.3390/jof3030033
dc.identifier.endpage12
dc.identifier.issn2309-608X
dc.identifier.issued3
dc.identifier.journaltitleJournal of Fungien
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/4874
dc.identifier.volume3
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttp://www.mdpi.com/2309-608X/3/3/33
dc.rights© 2017, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectFungal pigmentsen
dc.subjectPerstractionen
dc.subjectSurfactanten
dc.subjectTalaromycesen
dc.titlePerstraction of intracellular pigments through submerged fermentation of Talaromyces spp. in a surfactant rich media: a novel approach for enhanced pigment recoveryen
dc.typeArticle (peer-reviewed)en
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