Perstraction of intracellular pigments through submerged fermentation of Talaromyces spp. in a surfactant rich media: a novel approach for enhanced pigment recovery
dc.contributor.author | Morales-Oyervides, Lourdes | |
dc.contributor.author | Oliveira, Jorge C. | |
dc.contributor.author | Sousa-Gallagher, Maria J. | |
dc.contributor.author | Méndez-Zavala, Alejandro | |
dc.contributor.author | Montañez, Julio | |
dc.contributor.funder | Consejo Nacional de Ciencia y Tecnología | |
dc.date.accessioned | 2017-10-18T09:40:11Z | |
dc.date.available | 2017-10-18T09:40:11Z | |
dc.date.issued | 2017 | |
dc.description.abstract | A high percentage of the pigments produced by Talaromyces spp. remains inside the cell, which could lead to a high product concentration inhibition. To overcome this issue an extractive fermentation process, perstraction, was suggested, which involves the extraction of the intracellular products out of the cell by using a two-phase system during the fermentation. The present work studied the effect of various surfactants on secretion of intracellular pigments produced by Talaromyces spp. in submerged fermentation. Surfactants used were: non-ionic surfactants (Tween 80, Span 20 and Triton X-100) and a polyethylene glycerol polymer 8000, at different concentrations (5, 20, 35 g/L). The highest extracellular pigment yield (16 OD500nm) was reached using Triton X-100 (35 g/L), which was 44% higher than the control (no surfactant added). The effect of addition time of the selected surfactant was further studied. The highest extracellular pigment concentration (22 OD500nm) was achieved when the surfactant was added at 120 h of fermentation. Kinetics of extracellular and intracellular pigments were examined. Total pigment at the end of the fermentation using Triton X-100 was 27.7% higher than the control, confirming that the use of surfactants partially alleviated the product inhibition during the pigment production culture | en |
dc.description.sponsorship | Consejo Nacional de Ciencia y Tecnología (215490-2011) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Published Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.articleid | 33 | |
dc.identifier.citation | Morales-Oyervides, L., Oliveira, J., Sousa-Gallagher, M., Méndez-Zavala, A. and Montañez, J. (2017) 'Perstraction of intracellular pigments through submerged fermentation of Talaromyces spp. in a surfactant rich media: a novel approach for enhanced pigment recovery', Journal of Fungi, 3(3), 33 (12pp). doi: 10.3390/jof3030033 | en |
dc.identifier.doi | 10.3390/jof3030033 | |
dc.identifier.endpage | 12 | |
dc.identifier.issn | 2309-608X | |
dc.identifier.issued | 3 | |
dc.identifier.journaltitle | Journal of Fungi | en |
dc.identifier.startpage | 1 | |
dc.identifier.uri | https://hdl.handle.net/10468/4874 | |
dc.identifier.volume | 3 | |
dc.language.iso | en | en |
dc.publisher | MDPI | en |
dc.relation.uri | http://www.mdpi.com/2309-608X/3/3/33 | |
dc.rights | © 2017, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Fungal pigments | en |
dc.subject | Perstraction | en |
dc.subject | Surfactant | en |
dc.subject | Talaromyces | en |
dc.title | Perstraction of intracellular pigments through submerged fermentation of Talaromyces spp. in a surfactant rich media: a novel approach for enhanced pigment recovery | en |
dc.type | Article (peer-reviewed) | en |
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