Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model

dc.contributor.authorStrain, Conall R.
dc.contributor.authorCollins, Kenneth C.
dc.contributor.authorNaughton, Violetta
dc.contributor.authorMcSorley, Emeir M.
dc.contributor.authorStanton, Catherine
dc.contributor.authorSmyth, Thomas J.
dc.contributor.authorSoler-Vila, Anna
dc.contributor.authorRea, Mary C.
dc.contributor.authorRoss, R. Paul
dc.contributor.authorCherry, Paul
dc.contributor.authorAllsopp, Philip J.
dc.contributor.funderMarine Instituteen
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.date.accessioned2019-11-23T06:33:44Z
dc.date.available2019-11-23T06:33:44Z
dc.date.issued2019-02-25
dc.description.abstractBackground: Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate. These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.Methods: This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID. Results: Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h). Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h. The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05). Furthermore, the acetate:propionate ratio was significantly reduced (p < 0.05) for both CD and DE following 24, 36 and 48 h fermentation. Conclusion: The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivoen
dc.description.sponsorshipMFFRI/07/01en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationStrain, C.R., Collins, K.C., Naughton, V., McSorley, E.M., Stanton, C., Smyth, T.J., Soler-Vila, A., Rea, M.C., Ross, P.R., Cherry, P. and Allsopp, P.J., 2019. Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model. European journal of nutrition, (17pp). DOI:10.1007/s00394-019-01909-6en
dc.identifier.doi10.1007/s00394-019-01909-6en
dc.identifier.eissn1436-6215
dc.identifier.endpage17en
dc.identifier.issn1436-6207
dc.identifier.journaltitleEuropean Journal of Nutritionen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9187
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urihttps://link.springer.com/article/10.1007%2Fs00394-019-01909-6
dc.rights© The Author(s) 2019en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectSeaweedsen
dc.subjectMacroalgaeen
dc.subjectLaminaria digitataen
dc.subjectDietary fiberen
dc.subjectPrebioticen
dc.subjectGut microbiotaen
dc.subjectShort-chain fatty acidsen
dc.subjectMetagenomicsen
dc.titleEffects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic modelen
dc.typeArticle (peer-reviewed)en
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