Protective role of exogenous phytohormones on redox status in pea seedlings under copper stress

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dc.contributor.authorBen Massoud, Marouane
dc.contributor.authorSakouhi, Lamia
dc.contributor.authorKarmous, Inès
dc.contributor.authorZhu, Yao
dc.contributor.authorEl Ferjani, Ezzedine
dc.contributor.authorSheehan, David
dc.contributor.authorChaoui, Abdelilah
dc.contributor.funderTunisian Ministry of Higher Education, Scientific Research and Technologyen
dc.contributor.funderUniversity College Corken
dc.date.accessioned2018-04-24T11:39:04Z
dc.date.available2018-04-24T11:39:04Z
dc.date.issued2017-12-09
dc.date.updated2018-04-24T10:49:53Z
dc.description.abstractThe present work aims to provide insight on the role of phytohormone application in developing efficient practical defense strategies to improve plants tolerance under heavy metal contamination. For this purpose, pea (Pisum sativum L.) seeds were germinated in an aqueous solution of 200 mu M CuCl2 up to the 3rd day and then continued to germinate in the presence of distilled water (stress cessation) or were subjected to following combinations: Cu + 1 mu M IAA and Cu + 1 mu M GA(3) for 3 additional days. The results showed that copper excess induced oxidative stress in germinating seeds, which resulted in changes of the redox state of glutathione and cysteine, and proteomics revealed Cu-induced modifications of thiols (SH) and carbonyls (CO) (indicators of protein oxidation). However, application of IAA or GA(3) in the germination medium after 3 days of Cu exposure alleviated toxicity on seedlings, despite the persistence of Cu up to 6th day. This improving effect seems to be mediated by a cell Cu accumulation decrease and a protein reduced status recovery, since phytohormones modulate thioredoxin/ferredoxin systems in favor of protecting proteins against oxidation. In addition, an IAA and GA(3) protective effect was evidenced by a cellular homeostasis amelioration resulting from the balance conservation between the regeneration and consumption processes of glutathione and cysteine reduced forms. The exogenous effectors also induced modifications of profiles of SH and CO, suggesting changes in the regulation and expression of proteins that could be involved in defense mechanism against Cu stress.en
dc.description.sponsorshipUniversity College Cork (School of Biochemistry and Cell Biology & Environmental Research Institute)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBen Massoud, M., Sakouhi, L., Karmous, I., Zhu, Y., El Ferjani, E., Sheehan, D. and Chaoui, A. (2018) 'Protective role of exogenous phytohormones on redox status in pea seedlings under copper stress'. Journal of Plant Physiology, 221, pp. 51-61. doi:10.1016/j.jplph.2017.11.014en
dc.identifier.doi10.1016/j.jplph.2017.11.014
dc.identifier.endpage61en
dc.identifier.issn0176-1617
dc.identifier.journaltitleJournal of Plant Physiologyen
dc.identifier.startpage51en
dc.identifier.urihttps://hdl.handle.net/10468/5865
dc.identifier.volume221en
dc.language.isoenen
dc.rights© 2017, Elsevier GmbH. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectAlleviates cadmium toxicityen
dc.subjectOxidative stressen
dc.subjectArabidopsis-thalianaen
dc.subjectThioredoxin reductaseen
dc.subjectGlutathione-reductaseen
dc.subjectNitric-oxideen
dc.subjectAciden
dc.subjectToleranceen
dc.subjectAscorbateen
dc.subjectCysteineen
dc.titleProtective role of exogenous phytohormones on redox status in pea seedlings under copper stressen
dc.typeArticle (peer-reviewed)en
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