Caste- and pesticide-specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees
dc.contributor.author | Colgan, Thomas J. | |
dc.contributor.author | Fletcher, Isabel K. | |
dc.contributor.author | Arce, Andres N. | |
dc.contributor.author | Gill, Richard J. | |
dc.contributor.author | Ramos Rodrigues, Ana | |
dc.contributor.author | Stolle, Eckart | |
dc.contributor.author | Chittka, Lars | |
dc.contributor.author | Wurm, Yannick | |
dc.contributor.funder | European Research Council | en |
dc.contributor.funder | Biotechnology and Biological Sciences Research Council | en |
dc.contributor.funder | Engineering and Physical Sciences Research Council | en |
dc.date.accessioned | 2019-11-20T06:01:36Z | |
dc.date.available | 2019-11-20T06:01:36Z | |
dc.date.issued | 2019-03-06 | |
dc.description.abstract | Social bees are important insect pollinators of wildflowers and agricultural crops, making their reported declines a global concern. A major factor implicated in these declines is the widespread use of neonicotinoid pesticides. Indeed, recent research has demonstrated that exposure to low doses of these neurotoxic pesticides impairs bee behaviours important for colony function and survival. However, our understanding of the molecular-genetic pathways that lead to such effects is limited, as is our knowledge of how effects may differ between colony members. To understand what genes and pathways are affected by exposure of bumblebee workers and queens to neonicotinoid pesticides, we implemented a transcriptome-wide gene expression study. We chronically exposed Bombus terrestriscolonies to either clothianidin or imidacloprid at field-realistic concentrations while controlling for factors including colony social environment and worker age. We reveal that genes involved in important biological processes including mitochondrial function are differentially expressed in response to neonicotinoid exposure. Additionally, clothianidin exposure had stronger effects on gene expression amplitude and alternative splicing than imidacloprid. Finally, exposure affected workers more strongly than queens. Our work demonstrates how RNA-Seq transcriptome profiling can provide detailed novel insight on the mechanisms mediating pesticide toxicity to a key insect pollinator. | en |
dc.description.sponsorship | K000128 | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Published Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.citation | Colgan, T.J., Fletcher, I.K., Arce, A.N., Gill, R.J., Ramos Rodrigues, A., Stolle, E., Chittka, L. and Wurm, Y. (2019) 'Caste‐and pesticide‐specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees'. Molecular ecology, 28(8), pp.1964-1974 doi:10.1111/mec.15047 | en |
dc.identifier.doi | 10.1111/mec.15047 | en |
dc.identifier.eissn | 1365-294X | |
dc.identifier.endpage | 1974 | en |
dc.identifier.issn | 0962-1083 | |
dc.identifier.issued | 8 | en |
dc.identifier.journaltitle | Molecular Ecology | en |
dc.identifier.startpage | 1964 | en |
dc.identifier.uri | https://hdl.handle.net/10468/9147 | |
dc.identifier.volume | 28 | en |
dc.language.iso | en | en |
dc.publisher | Wiley | en |
dc.relation.project | info:eu-repo/grantAgreement/RCUK/NERC/NE/L00626X/1/GB/Behavioural and molecular responses to pesticide exposure in bumblebees./ | en |
dc.relation.project | info:eu-repo/grantAgreement/RCUK/NERC/NE/L00755X/1/GB/Behavioural and molecular responses to pesticide exposure in bumblebees/ | en |
dc.relation.project | info:eu-repo/grantAgreement/RCUK/BBSRC/BB/M009513/1/GB/London Interdisciplinary Doctoral Programme/ | en |
dc.relation.project | info:eu-repo/grantAgreement/RCUK/BBSRC/BB/K004204/1/GB/Genome Annotation for the Masses/ | en |
dc.relation.uri | https://onlinelibrary.wiley.com/doi/full/10.1111/mec.15047 | |
dc.rights | © 2019 The Authors. Molecular Ecology Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | Ecotoxicology, | en |
dc.subject | Molecular diagnostics | en |
dc.subject | Neonicotinoid insecticides | en |
dc.subject | Nicotinic acetylcholine receptors | en |
dc.subject | Pollinator health | en |
dc.subject | Xenobiotics | en |
dc.title | Caste- and pesticide-specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees | en |
dc.type | Article (peer-reviewed) | en |
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