Caste- and pesticide-specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees

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dc.contributor.authorColgan, Thomas J.
dc.contributor.authorFletcher, Isabel K.
dc.contributor.authorArce, Andres N.
dc.contributor.authorGill, Richard J.
dc.contributor.authorRamos Rodrigues, Ana
dc.contributor.authorStolle, Eckart
dc.contributor.authorChittka, Lars
dc.contributor.authorWurm, Yannick
dc.contributor.funderEuropean Research Councilen
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen
dc.contributor.funderEngineering and Physical Sciences Research Councilen
dc.date.accessioned2019-11-20T06:01:36Z
dc.date.available2019-11-20T06:01:36Z
dc.date.issued2019-03-06
dc.description.abstractSocial bees are important insect pollinators of wildflowers and agricultural crops, making their reported declines a global concern. A major factor implicated in these declines is the widespread use of neonicotinoid pesticides. Indeed, recent research has demonstrated that exposure to low doses of these neurotoxic pesticides impairs bee behaviours important for colony function and survival. However, our understanding of the molecular-genetic pathways that lead to such effects is limited, as is our knowledge of how effects may differ between colony members. To understand what genes and pathways are affected by exposure of bumblebee workers and queens to neonicotinoid pesticides, we implemented a transcriptome-wide gene expression study. We chronically exposed Bombus terrestriscolonies to either clothianidin or imidacloprid at field-realistic concentrations while controlling for factors including colony social environment and worker age. We reveal that genes involved in important biological processes including mitochondrial function are differentially expressed in response to neonicotinoid exposure. Additionally, clothianidin exposure had stronger effects on gene expression amplitude and alternative splicing than imidacloprid. Finally, exposure affected workers more strongly than queens. Our work demonstrates how RNA-Seq transcriptome profiling can provide detailed novel insight on the mechanisms mediating pesticide toxicity to a key insect pollinator.en
dc.description.sponsorshipK000128en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationColgan, T.J., Fletcher, I.K., Arce, A.N., Gill, R.J., Ramos Rodrigues, A., Stolle, E., Chittka, L. and Wurm, Y. (2019) 'Caste‐and pesticide‐specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees'. Molecular ecology, 28(8), pp.1964-1974 doi:10.1111/mec.15047en
dc.identifier.doi10.1111/mec.15047en
dc.identifier.eissn1365-294X
dc.identifier.endpage1974en
dc.identifier.issn0962-1083
dc.identifier.issued8en
dc.identifier.journaltitleMolecular Ecologyen
dc.identifier.startpage1964en
dc.identifier.urihttps://hdl.handle.net/10468/9147
dc.identifier.volume28en
dc.language.isoenen
dc.publisherWileyen
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/NERC/NE/L00626X/1/GB/Behavioural and molecular responses to pesticide exposure in bumblebees./en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/NERC/NE/L00755X/1/GB/Behavioural and molecular responses to pesticide exposure in bumblebees/en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/BBSRC/BB/M009513/1/GB/London Interdisciplinary Doctoral Programme/en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/BBSRC/BB/K004204/1/GB/Genome Annotation for the Masses/en
dc.relation.urihttps://onlinelibrary.wiley.com/doi/full/10.1111/mec.15047
dc.rights© 2019 The Authors. Molecular Ecology Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectEcotoxicology,en
dc.subjectMolecular diagnosticsen
dc.subjectNeonicotinoid insecticidesen
dc.subjectNicotinic acetylcholine receptorsen
dc.subjectPollinator healthen
dc.subjectXenobioticsen
dc.titleCaste- and pesticide-specific effects of neonicotinoid pesticide exposure on gene expression in bumblebeesen
dc.typeArticle (peer-reviewed)en
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