Dietary a-lactalbumin alters energy balance, gut microbiota composition and intestinal nutrient transporter expression in high-fat diet-fed mice

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dc.contributor.authorBoscaini, Serena
dc.contributor.authorCabrera-Rubio, Raul
dc.contributor.authorSpeakman, John R.
dc.contributor.authorCotter, Paul D.
dc.contributor.authorCryan, John F.
dc.contributor.authorNilaweera, Kanishka N.
dc.contributor.funderTeagascen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-08-22T13:35:36Z
dc.date.available2019-08-22T13:35:36Z
dc.date.issued2019-05-28
dc.date.updated2019-08-22T13:22:46Z
dc.description.abstractRecently there has been a considerable rise in the frequency of metabolic diseases, such as obesity, due to changes in lifestyle and resultant imbalances between energy intake and expenditure. Whey proteins are considered as potentially important components of a dietary solution to the obesity problem. However, the roles of individual whey proteins in energy balance remain poorly understood. This study investigated the effects of a high-fat diet (HFD) containing α-lactalbumin (LAB), a specific whey protein, or the non-whey protein casein (CAS), on energy balance, nutrient transporters expression and enteric microbial populations. C57BL/6J mice (n 8) were given an HFD containing either 20 % CAS or LAB as protein sources or a low-fat diet containing CAS for 10 weeks. HFD-LAB-fed mice showed a significant increase in cumulative energy intake (P=0·043), without differences in body weight, energy expenditure, locomotor activity, RER or subcutaneous and epididymal white adipose tissue weight. HFD-LAB intake led to a decrease in the expression of glut2 in the ileum (P=0·05) and in the fatty acid transporter cd36 (P<0·001) in both ileum and jejunum. This suggests a reduction in absorption efficiency within the small intestine in the HFD-LAB group. DNA from faecal samples was used for 16S rRNA-based assessment of intestinal microbiota populations; the genera Lactobacillus, Parabacteroides and Bifidobacterium were present in significantly higher proportions in the HFD-LAB group. These data indicate a possible functional relationship between gut microbiota, intestinal nutrient transporters and energy balance, with no impact on weight gain.en
dc.description.sponsorshipTeagasc (Teagasc Walsh Fellowship scheme (grant number 2016007)); Science Foundation Ireland ((SFI) under grant number SFI/16/BBSRC/3389 and BBSRC under the grant number BB/P009875/1)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBoscaini, S., Cabrera-Rubio, R., Speakman, J. R., Cotter, P. D., Cryan, J. F. and Nilaweera, K. N. (2019) 'Dietary α-lactalbumin alters energy balance, gut microbiota composition and intestinal nutrient transporter expression in high-fat diet-fed mice', British Journal of Nutrition, 121(10), pp. 1097-1107. doi: 10.1017/S0007114519000461en
dc.identifier.doi10.1017/S0007114519000461en
dc.identifier.endpage1107en
dc.identifier.issn0007-1145
dc.identifier.issued10en
dc.identifier.journaltitleBritish Journal of Nutritionen
dc.identifier.startpage1097en
dc.identifier.urihttps://hdl.handle.net/10468/8380
dc.identifier.volume121en
dc.language.isoenen
dc.publisherCambridge University Press (CUP)en
dc.relation.projectinfo:eu-repo/grantAgreement/RCUK/BBSRC/BB/P009875/1/GB/Bilateral BBSRC-SFI: The role of hypothalamic neuropeptide network in regulating tissue sizes in response to diet energy content and composition/en
dc.relation.urihttps://doi.org/10.1017/S0007114519000461
dc.rights© The Authors 2019. Published by Cambridge University Press on behalf of the Nutrition Society.en
dc.subjectDietary a-lactalbuminen
dc.subjectEnergy balanceen
dc.subjectGut microbiotaen
dc.subjectNutrient transporter expressionen
dc.subjectWhey proteinsen
dc.subjectHigh-fat dieten
dc.subjectCaseinen
dc.subjectCluster differentiation 36en
dc.subjectEpididymal white adipose tissueen
dc.subjectFatty acid synthaseen
dc.subjectFatty acid transporter protein 4en
dc.subjecta-lactalbuminen
dc.subjectLactoferrinen
dc.subjectLow-fat dieten
dc.subjectNeuropeptide Yen
dc.subjectProopiomelanocortinen
dc.subjectSubcutaneous white adipose tissueen
dc.subjectWhey protein isolateen
dc.titleDietary a-lactalbumin alters energy balance, gut microbiota composition and intestinal nutrient transporter expression in high-fat diet-fed miceen
dc.typeArticle (peer-reviewed)en
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