dc.contributor.author	Hayes, Stephen
dc.contributor.author	Vincentelli, Renaud
dc.contributor.author	Mahony, Jennifer
dc.contributor.author	Nauta, Arjen
dc.contributor.author	Ramond, Laurie
dc.contributor.author	Lugli, Gabriele A.
dc.contributor.author	Ventura, Marco
dc.contributor.author	van Sinderen, Douwe
dc.contributor.author	Cambillau, Christian
dc.contributor.funder	Irish Research Council	en
dc.contributor.funder	Science Foundation Ireland	en
dc.contributor.funder	French Infrastructure for Integrated Structural Biology	en
dc.date.accessioned	2018-09-20T09:10:29Z
dc.date.available	2018-09-20T09:10:29Z
dc.date.issued	2018-09-11
dc.date.updated	2018-09-20T08:59:52Z
dc.description.abstract	With increasing numbers of 3D structures of bacteriophage components, combined with powerful in silico predictive tools, it has become possible to decipher the structural assembly and associated functionality of phage adhesion devices. Recently, decorations have been reported in the tail and neck passage structures of members of the so‐called 936 group of lactococcal siphophages. In the current report, using bioinformatic analysis we identified a conserved carbohydrate binding module (CBM) among many of the virion baseplate Dit components, in addition to the CBM present in the ‘classical’ receptor binding proteins (RBPs). We observed that, within these so‐called ‘evolved’ Dit proteins, the identified CBMs have structurally conserved folds, yet can be grouped into four distinct classes. We expressed such modules in fusion with GFP, and demonstrated their binding capability to their specific host using fluorescent binding assays with confocal microscopy. We detected evolved Dits in several phages infecting various Gram‐positive bacterial species, including mycobacteria. The omnipresence of CBM domains in Siphophages indicates their auxiliary role in infection, as they can assist in the specific recognition of and attachment to their host, thus ensuring a highly efficient and specific phage‐host adhesion process as a prelude to DNA injection.	en
dc.description.sponsorship	Irish Research Council (Industry Partnership Studentship); French Infrastructure for Integrated Structural Biology (ANR-10-INSB-05-01)	en
dc.description.status	Peer reviewed	en
dc.description.version	Accepted Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Hayes, S., Vincentelli, R., Mahony, J., Nauta, A., Ramond, L., Lugli, G. A., Ventura, M., van Sinderen, D. and Cambillau, C. (2018) 'Functional carbohydrate binding modules identified in evolved Dits from siphophages infecting various Gram-positive bacteria', Molecular Microbiology. doi:10.1111/mmi.14124	en
dc.identifier.doi	10.1111/mmi.14124
dc.identifier.issn	0950-382X
dc.identifier.issn	1365-2958
dc.identifier.journaltitle	Molecular Microbiology	en
dc.identifier.uri	https://hdl.handle.net/10468/6822
dc.language.iso	en	en
dc.publisher	John Wiley & Sons, Inc.	en
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/	en
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/	en
dc.rights	© 2018, John Wiley & Sons Inc. This is the peer reviewed version of the following article: Hayes, S., Vincentelli, R., Mahony, J., Nauta, A., Ramond, L., Lugli, G. A., Ventura, M., van Sinderen, D. and Cambillau, C. (2018) 'Functional carbohydrate binding modules identified in evolved Dits from siphophages infecting various Gram-positive bacteria', Molecular Microbiology. doi:10.1111/mmi.14124, which has been published in final form at https://doi.org/10.1111/mmi.14124. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.	en
dc.subject	Bacteriophage	en
dc.subject	Lactococcus lactis	en
dc.subject	Receptor-binding protein	en
dc.subject	Distal tail protein	en
dc.subject	Carbohydrate binding module	en
dc.subject	Phage-host interactions	en
dc.title	Functional carbohydrate binding modules identified in evolved Dits from siphophages infecting various Gram-positive bacteria	en
dc.type	Article (peer-reviewed)	en

Functional carbohydrate binding modules identified in evolved Dits from siphophages infecting various Gram-positive bacteria

Files

Original bundle

License bundle

Collections