Characterization of an endolysin targeting Clostridioides difficile that affects spore outgrowth.

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dc.contributor.authorMondal, Shakhinur Islamen
dc.contributor.authorAkter, Arzubaen
dc.contributor.authorDraper, Lorraine A.en
dc.contributor.authorRoss, R. Paulen
dc.contributor.authorHill, Colinen
dc.contributor.funderHorizon 2020en
dc.date.accessioned2023-05-31T11:30:33Z
dc.date.available2023-05-31T11:30:33Z
dc.date.issued2021-05-26T00:00:00Zen
dc.description.abstractClostridioides difficile is a spore-forming enteric pathogen causing life-threatening diarrhoea and colitis. Microbial disruption caused by antibiotics has been linked with susceptibility to, and transmission and relapse of, C. difficile infection. Therefore, there is an urgent need for novel therapeutics that are effective in preventing C. difficile growth, spore germination, and outgrowth. In recent years bacteriophage-derived endolysins and their derivatives show promise as a novel class of antibacterial agents. In this study, we recombinantly expressed and characterized a cell wall hydrolase (CWH) lysin from C. difficile phage, phiMMP01. The full-length CWH displayed lytic activity against selected C. difficile strains. However, removing the N-terminal cell wall binding domain, creating CWH351—656, resulted in increased and/or an expanded lytic spectrum of activity. C. difficile specificity was retained versus commensal clostridia and other bacterial species. As expected, the putative cell wall binding domain, CWH1—350, was completely inactive. We also observe the effect of CWH351—656 on preventing C. difficile spore outgrowth. Our results suggest that CWH351—656 has therapeutic potential as an antimicrobial agent against C. difficile infection.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid5690en
dc.identifier.citationMondal, S. I., Akter, A., Draper, L. A., Ross, R. P. and Hill, C. (2021) 'Characterization of an endolysin targeting Clostridioides difficile that affects spore outgrowth', International Journal of Molecular Sciences, 22(11), 5690 (14pp). doi: 10.3390/ijms22115690en
dc.identifier.doi10.3390/ijms22115690en
dc.identifier.eissn1422-0067en
dc.identifier.endpage14en
dc.identifier.issn1661-6596en
dc.identifier.issued11en
dc.identifier.journaltitleInternational Journal of Molecular Sciencesen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14530
dc.identifier.volume22en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-COFUND-FP/754535/EU/APC Postdoctoral EXcellence Programme/APEXen
dc.rights© 2021, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectClostridioides difficile infectionen
dc.subjectAntibioticsen
dc.subjectAntimicrobial agenten
dc.subjectAntimicrobial resistanceen
dc.subjectEndolysinen
dc.subjectEnteric pathogenen
dc.titleCharacterization of an endolysin targeting Clostridioides difficile that affects spore outgrowth.en
dc.typeArticle (peer-reviewed)en
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