Comparing in vitro faecal fermentation methods as surrogates for phage therapy application

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dc.contributor.authorÁcs, Norberten
dc.contributor.authorHolohan, Rossen
dc.contributor.authorDunne, Laura J.en
dc.contributor.authorFernandes, Adrian R.en
dc.contributor.authorClooney, Adam G.en
dc.contributor.authorDraper, Lorraine A.en
dc.contributor.authorRoss, R. Paulen
dc.contributor.authorHill, Colinen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2024-04-04T15:46:53Z
dc.date.available2024-04-04T15:46:53Z
dc.date.issued2022en
dc.description.abstractThe human microbiome and its importance in health and disease have been the subject of numerous research articles. Most microbes reside in the digestive tract, with up to 1012 cells per gram of faecal material found in the colon. In terms of gene number, it has been estimated that the gut microbiome harbours >100 times more genes than the human genome. Several human intestinal diseases are strongly associated with disruptions in gut microbiome composition. Less studied components of the gut microbiome are the bacterial viruses called bacteriophages that may be present in numbers equal to or greater than the prokaryotes. Their potential to lyse their bacterial hosts, or to act as agents of horizontal gene transfer makes them important research targets. In this study in vitro faecal fermentation systems were developed and compared for their ability to act as surrogates for the human colon. Changes in bacterial and viral composition occurred after introducing a high-titre single phage preparation both with and without a known bacterial host during the 24 h-long fermentation. We also show that during this timeframe 50 mL plastic tubes can provide data similar to that generated in a sophisticated faecal fermenter system. This knowledge can guide us to a better understanding of the short-term impact of bacteriophage transplants on the bacteriomes and viromes of human recipients.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid2632en
dc.identifier.citationÁcs, N., Holohan, R., Dunne, L.J., Fernandes, A.R., Clooney, A.G., Draper, L.A., Ross, R.P. and Hill, C. (2022) ‘Comparing in vitro faecal fermentation methods as surrogates for phage therapy application’, Viruses, 14(12), 2632 (13pp). doi: 10.3390/v14122632en
dc.identifier.doi10.3390/v14122632en
dc.identifier.eissn1999-4915en
dc.identifier.endpage13en
dc.identifier.issued12en
dc.identifier.journaltitleVirusesen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15769
dc.identifier.volume14en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI ERC Development Programme/15/ERCD/3189/IE/GUTPHAGE: bacteriophage as agents to manipulate the microbiota for human health/en
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriophageen
dc.subjectPhageomeen
dc.subjectBacteriomeen
dc.subjectHuman gut microbiomeen
dc.subjectFaecal fermentationen
dc.titleComparing in vitro faecal fermentation methods as surrogates for phage therapy applicationen
dc.typeArticle (peer-reviewed)en
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