Isolation and characterization of a novel lytic Parabacteroides distasonis bacteriophage φPDS1 from the human gut

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dc.contributor.authorCortés-Martín, Adriánen
dc.contributor.authorDenise, Rémien
dc.contributor.authorGuerin, Emmaen
dc.contributor.authorStockdale, Stephen R.en
dc.contributor.authorDraper, Lorraine A.en
dc.contributor.authorRoss, R. Paulen
dc.contributor.authorShkoporov, Andrey N.en
dc.contributor.authorHill, Colinen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2024-04-18T15:28:33Z
dc.date.available2024-04-18T15:28:33Z
dc.date.issued2024en
dc.description.abstractThe human gut microbiome plays a significant role in health and disease. The viral component (virome) is predominantly composed of bacteriophages (phages) and has received significantly less attention in comparison to the bacteriome. This knowledge gap is largely due to challenges associated with the isolation and characterization of novel gut phages, and bioinformatic hurdles such as the lack of a universal phage marker gene and the absence of sufficient numbers of homologs in viral databases. Here, we describe the isolation from human feces of a novel lytic phage with siphovirus morphology, φPDS1, infecting Parabacteroides distasonis APCS2/PD, and classified within a newly proposed Sagittacolavirus genus. In silico and biological characterization of this phage is presented in this study. Key to the isolation of φPDS1 was the antibiotic-driven selective enrichment of the bacterial host in a fecal fermenter. Despite producing plaques and lacking genes associated with lysogeny, φPDS1 demonstrates the ability to coexist in liquid culture for multiple days without affecting the abundance of its host. Multiple studies have shown that changes in Parabacteroides distasonis abundance can be linked to various disease states, rendering this novel phage-host pair and their interactions of particular interest.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationCortés-Martín, A., Denise, R., Guerin, E., Stockdale, S.R., Draper, L.A., Ross, R.P., Shkoporov, A.N. and Hill, C. (2024) ‘Isolation and characterization of a novel lytic Parabacteroides distasonis bacteriophage φPDS1 from the human gut’, Gut Microbes, 16(1), 2298254 (24pp). doi: 10.1080/19490976.2023.2298254en
dc.identifier.doi10.1080/19490976.2023.2298254en
dc.identifier.eissn1949-0984en
dc.identifier.endpage24en
dc.identifier.issn1949-0976en
dc.identifier.issued1en
dc.identifier.journaltitleGut Microbesen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15815
dc.identifier.volume16en
dc.language.isoenen
dc.publisherTaylor & Francisen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacteriophageen
dc.subjectParabacteroidesen
dc.subjectPhage- bacteria interactionen
dc.subjectPhage isolationen
dc.subjectFecal fermentationen
dc.subjectGut microbiomeen
dc.subjectPhage characterizationen
dc.titleIsolation and characterization of a novel lytic Parabacteroides distasonis bacteriophage φPDS1 from the human guten
dc.typeArticle (peer-reviewed)en
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