The two peptide lantibiotic lacticin 3147 acts synergistically with polymyxin to inhibit Gram negative bacteri

dc.contributor.authorDraper, Lorraine A.
dc.contributor.authorCotter, Paul D.
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2016-02-08T13:30:34Z
dc.date.available2016-02-08T13:30:34Z
dc.date.issued2013-09-26
dc.description.abstractBackground: The emergence of bacterial drug resistance encourages the re-evaluation of the potential of existing antimicrobials. Lantibiotics are post-translationally modified, ribosomally synthesised antimicrobial peptides with a broad spectrum antimicrobial activity. Here, we focussed on expanding the potential of lacticin 3147, one of the most studied lantibiotics and one which possesses potent activity against a wide range of Gram positive species including many nosocomial pathogens. More specifically, our aim was to investigate if lacticin 3147 activity could be enhanced when combined with a range of different clinical antibiotics. Results: Initial screening revealed that polymyxin B and polymyxin E (colistin) exhibited synergistic activity with lacticin 3147. Checkerboard assays were performed against a number of strains, including both Gram positive and Gram negative species. The resultant fractional inhibitory concentration (FIC) index values established that, while partial synergy was detected against Gram positive targets, synergy was obvious against Gram negative species, including Cronobacter and E. coli. Conclusions: Combining lacticin 3147 with low levels of a polymyxin could provide a means of broadening target specificity of the lantibiotic, while also reducing polymyxin use due to the lower concentrations required as a result of synergy.en
dc.description.sponsorshipScience Foundation Ireland (Investigator Award 10/IN.1/B3027)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid212
dc.identifier.citationDRAPER, L. A., COTTER, P. D., HILL, C. & ROSS, R. P. 2013. The two peptide lantibiotic lacticin 3147 acts synergistically with polymyxin to inhibit Gram negative bacteria. BMC Microbiology, 13:212, 1-8. http://dx.doi.org/10.1186/1471-2180-13-212en
dc.identifier.doi10.1186/1471-2180-13-212
dc.identifier.endpage8en
dc.identifier.issn1471-2180
dc.identifier.journaltitleBMC Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/2264
dc.identifier.volume13en
dc.language.isoenen
dc.publisherBioMed Central Ltd.en
dc.rights© Draper et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://​creativecommons.​org/​licenses/​by/​2.​0en
dc.subjectAntimicrobialen
dc.subjectSynergyen
dc.subjectLantibioticen
dc.subjectBacteriocinen
dc.subjectLacticin 3147en
dc.subjectPolymyxinen
dc.titleThe two peptide lantibiotic lacticin 3147 acts synergistically with polymyxin to inhibit Gram negative bacterien
dc.typeArticle (peer-reviewed)en
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