Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways

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dc.contributor.authorJames, Kieran
dc.contributor.authorO'Connell Motherway, Mary
dc.contributor.authorBottacini, Francesca
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderIrish Research Councilen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHealth Research Boarden
dc.date.accessioned2017-01-09T10:48:25Z
dc.date.available2017-01-09T10:48:25Z
dc.date.issued2016-12-08
dc.date.updated2017-01-09T10:35:08Z
dc.description.abstractIn this study, we demonstrate that the prototype B. breve strain UCC2003 possesses specific metabolic pathways for the utilisation of lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNnT), which represent the central moieties of Type I and Type II human milk oligosaccharides (HMOs), respectively. Using a combination of experimental approaches, the enzymatic machinery involved in the metabolism of LNT and LNnT was identified and characterised. Homologs of the key genetic loci involved in the utilisation of these HMO substrates were identified in B. breve, B. bifidum, B. longum subsp. infantis and B. longum subsp. longum using bioinformatic analyses, and were shown to be variably present among other members of the Bifidobacterium genus, with a distinct pattern of conservation among human-associated bifidobacterial species.en
dc.description.sponsorshipIrish Research Council (Postgraduate Research Project Award: Project ID GOIPG/2013/651); Science Foundation Ireland (Grant No. SFI/12/RC/2273); Health Research Board (Postdoctoral Fellowship Grant No. PDTM/20011/9)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid38560
dc.identifier.citationJames, K., O’Connell Motherway, M., Bottacini, F. and van Sinderen, D. (2016) ‘Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways’, Scientific Reports, 6, 38560 (16pp). doi: 10.1038/srep38560en
dc.identifier.doi10.1038/srep38560
dc.identifier.endpage16en
dc.identifier.issn2045-2322
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/3448
dc.identifier.volume6en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rights© 2016, the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectLongum subsp. infantisen
dc.subjectDNA-microarray dataen
dc.subjectLactococcus lactisen
dc.subjectGut microbiotaen
dc.subjectMolecular cloningen
dc.subjectGene expressionen
dc.subjectNecrotizing enterocolitisen
dc.subjectBeta-galactosidasesen
dc.subjectEscherichia-colien
dc.subjectBifidumen
dc.titleBifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathwaysen
dc.typeArticle (peer-reviewed)en
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