Characterisation of a hydroxycinnamic acid esterase from the Bifidobacterium longum subsp. longum taxon

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dc.contributor.authorKelly, Sandra M.
dc.contributor.authorO'Callaghan, John
dc.contributor.authorKinsella, Mike
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-09-09T11:51:21Z
dc.date.available2019-09-09T11:51:21Z
dc.date.issued2018-11-09
dc.description.abstractBifidobacterium longum subsp. longum, a common member of the human gut microbiota with perceived positive health effects, is capable of metabolising certain complex plant carbohydrates which are typically found in the (adult) human diet. Potentially, these plant carbohydrates possibly could be employed to favourably modulate the microbial communities in the intestine. Hydroxycinnamic acids (HCAs) are plant phenolic compounds, attached to plant carbohydrates, which are associated with anti-oxidant and other beneficial properties. However, very little information is available regarding possible metabolism of HCA-containing glycans by bifidobacteria. In the current study, a gene encoding a hydroxycinnamic acid esterase was found to be conserved across the B. longum subsp. longum taxon and was present in a conserved locus associated with plant carbohydrate utilisation. The esterase was shown to be active against various HCA-containing substrates and was biochemically characterised in terms of substrate preference, and pH and temperature optima of the enzyme. Potentially, this novel hydroxycinnamic acid esterase could release HCAs from plant-based dietary sources, which may have benefits for the gut environment and thus host health.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid2690en
dc.identifier.citationKelly, S.M., O’Callaghan, J., Kinsella, M. and Van Sinderen, D., 2018. Characterisation of a hydroxycinnamic acid esterase from the Bifidobacterium longum subsp. longum taxon. Frontiers in Microbiology, 9 (2690). DOI:10.3389/fmicb.2018.02690en
dc.identifier.doi10.3389/fmicb.2018.02690en
dc.identifier.eissn1664-302X
dc.identifier.endpage14en
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8481
dc.identifier.volume9en
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/en
dc.relation.urihttps://www.frontiersin.org/article/10.3389/fmicb.2018.02690
dc.rights© 2018 Kelly, O’Callaghan, Kinsella and van Sinderenen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectEsteraseen
dc.subjectHydroxycinnamic aciden
dc.subjectFerulic aciden
dc.subjectBifidoacterium longumen
dc.subjectPlant phenolicsen
dc.subjectP- coumaric aciden
dc.subjectCaffeic aciden
dc.titleCharacterisation of a hydroxycinnamic acid esterase from the Bifidobacterium longum subsp. longum taxonen
dc.typeArticle (peer-reviewed)en
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