Positively charged, surfactant-free gold nanoparticles for nucleic acid delivery

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dc.contributor.authorGuo, Jianfeng
dc.contributor.authorArmstrong, Mark J.
dc.contributor.authorO'Driscoll, Caitríona M.
dc.contributor.authorHolmes, Justin D.
dc.contributor.authorRahme, Kamil
dc.contributor.funderIrish Research Council
dc.date.accessioned2016-01-26T12:10:16Z
dc.date.available2016-01-26T12:10:16Z
dc.date.issued2015-02-03
dc.description.abstractPositively charged, surfactant-free gold nanoparticles (Au NPs) with diameters ranging between 2-200 nm have been synthesised in water via a seed-mediated growth method, involving the reduction of gold(iii) chloride (AuCl3) by hydroxylamine hydrochloride (NH2OH[middle dot]HCl) in the presence of l-cysteine methyl ester hydrochloride (HSCH2CH(NH2)COOCH3[middle dot]HCl) as a capping agent. The mercapto group (-SH) on the capping ligand has a high affinity for Au, anchoring the cysteine group to the nanoparticles, whilst the ammonium group (-NH3+), formed by the presence of an amine group in slightly acidic media (pH [similar] 4.5-5), resulted in positively charged colloidal nanoparticles ([small zeta]-potential +33 to +49 mV), which was key to their electrostatic stability. Data from cytotoxicity studies performed on a range of different cell types (human and murine), including human prostate cancer cells (PC3), showed that the positively charged Au-l-cysteine-cysteine nanoparticles were less cytotoxic than positively charged Au NPs produced using commonly employed surfactant cetyl trimethyl ammonium bromide (CTAB) under similar conditions. In addition, the positively charged Au NPs could be successfully complexed with small interfering RNA (siRNA). At the cellular level, the uptake of fluorescein siRNA from the charged nanoparticles was comparable to uptake from the commercial carrier INTERFRin[trade mark sign], implying the potential application of these novel vectors for nucleic acid delivery.en
dc.description.sponsorshipIrish Research Council (Government of Ireland Postdoctoral Fellowship GOIPD/2013/150)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGUO, J., ARMSTRONG, M. J., O'DRISCOLL, C. M., HOLMES, J. D. & RAHME, K. 2015. Positively charged, surfactant-free gold nanoparticles for nucleic acid delivery. RSC Advances, 5, 17862-17871.http://dx.doi.org/10.1039/C4RA16294Cen
dc.identifier.doi10.1039/C4RA16294C
dc.identifier.endpage17871en
dc.identifier.issn2046-2069
dc.identifier.issued23en
dc.identifier.journaltitleRSC Advancesen
dc.identifier.startpage17862en
dc.identifier.urihttps://hdl.handle.net/10468/2216
dc.identifier.volume5en
dc.language.isoenen
dc.publisherRoyal Society of Chemistryen
dc.rights© 2015, the Authors. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectAmino acidsen
dc.subjectChlorine compoundsen
dc.subjectCytotoxicityen
dc.subjectGold alloysen
dc.subjectMetal nanoparticlesen
dc.subjectNanoparticlesen
dc.subjectNucleic acidsen
dc.subjectNucleic acid deliveriesen
dc.subjectCetyl trimethyl ammonium bromideen
dc.subjectCTABen
dc.subjectHuman prostate cancer cellsen
dc.subjectHydroxylamine hydrochlorideen
dc.subjectL-Cysteine methyl estersen
dc.subjectSeed-mediated growth methodsen
dc.subjectSmall interfering rna (siRNA)en
dc.subjectDiseasesen
dc.subjectDyesen
dc.subjectFiber optic sensorsen
dc.subjectMobile securityen
dc.subjectSurface active agentsen
dc.subjectElectrostatic stabilityen
dc.titlePositively charged, surfactant-free gold nanoparticles for nucleic acid deliveryen
dc.typeArticle (peer-reviewed)en
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