Nisin in combination with cinnamaldehyde and EDTA to control growth of Escherichia coli strains of swine origin

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dc.contributor.authorField, Des
dc.contributor.authorBaghou, Inès
dc.contributor.authorRea, Mary
dc.contributor.authorGardiner, Gillian E.
dc.contributor.authorRoss, R. Paul
dc.contributor.authorHill, Colin
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2018-02-06T13:36:29Z
dc.date.available2018-02-06T13:36:29Z
dc.date.issued2017
dc.description.abstractPost-weaning diarrhoea (PWD) due to enterotoxigenic Escherichia coli (ETEC) is an economically important disease in pig production worldwide. Although antibiotics have contributed significantly to mitigate the economic losses caused by PWD, there is major concern over the increased incidence of antimicrobial resistance among bacteria isolated from pigs. Consequently, suitable alternatives that are safe and effective are urgently required. Many naturally occurring compounds, including the antimicrobial peptide nisin and a number of plant essential oils, have been widely studied and are reported to be effective as antimicrobial agents against pathogenic microorganisms. Here, we evaluate the potential of nisin in combination with the essential oil cinnamaldehyde and ethylenediaminetetraacetic acid (EDTA) to control the growth of E. coli strains of swine origin including two characterized as ETEC. The results reveal that the use of nisin (10 μM) with low concentrations of trans-cinnamaldehyde (125 μg/mL) and EDTA (0.25–2%) resulted in extended lag phases of growth compared to when either antimicrobial is used alone. Further analysis through kill curves revealed that an approximate 1-log reduction in E. coli cell counts was observed against the majority of targets tested following 3 h incubation. These results highlight the potential benefits of combining the natural antimicrobial nisin with trans-cinnamaldehyde and EDTA as a new approach for the inhibition of E. coli strains of swine origin.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid35
dc.identifier.citationField, D., Baghou, I., Rea, M., Gardiner, G., Ross, R. and Hill, C. (2017) 'Nisin in combination with cinnamaldehyde and EDTA to control growth of Escherichia coli strains of swine origin', Antibiotics, 6(4), 35 (10pp). doi: 10.3390/antibiotics6040035en
dc.identifier.doi10.3390/antibiotics6040035
dc.identifier.endpage10
dc.identifier.issued4
dc.identifier.journaltitleAntibioticsen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/5400
dc.identifier.volume6
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Principal Investigator Programme (PI)/10/IN.1/B3027/IE/Lantibiotics: the next generation/
dc.relation.urihttp://www.mdpi.com/2079-6382/6/4/35
dc.rights© 2017, by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAntimicrobial resistanceen
dc.subjectAntibioticsen
dc.subjectAntimicrobial peptideen
dc.subjectEnterotoxigenic E. colien
dc.subjectNisinen
dc.subjectBacteriocinen
dc.subjectEssential oilen
dc.subjectCinnamaldehydeen
dc.subjectEDTAen
dc.titleNisin in combination with cinnamaldehyde and EDTA to control growth of Escherichia coli strains of swine originen
dc.typeArticle (peer-reviewed)en
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