Identification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitis

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dc.contributor.authorLynch, David
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorCotter, Paul D.
dc.contributor.authorHill, Colin
dc.contributor.authorField, Des
dc.contributor.authorBegley, Máire
dc.contributor.funderCork Institute of Technologyen
dc.date.accessioned2019-12-03T11:42:44Z
dc.date.available2019-12-03T11:42:44Z
dc.date.issued2019-10-16
dc.description.abstractOne hundred human-derived coagulase negative staphylococci (CoNS) were screened for antimicrobial activity using agar-based deferred antagonism assays with a range of indicator bacteria. Based on the findings of the screen and subsequent well assays with cell free supernatants and whole cell extracts, one strain, designated CIT060, was selected for further investigation. It was identified as Staphylococcus capitis and herein we describe the purification and characterisation of the novel bacteriocin that the strain produces. This bacteriocin which we have named capidermicin was extracted from the cell-free supernatant of S. capitis CIT060 and purified to homogeneity using reversed-phase high performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric (MS) analysis revealed that the capidermicin peptide has a mass of 5,464 Da. Minimal inhibitory concentration (MIC) experiments showed that capidermicin was active in the micro-molar range against all the Gram-positive bacteria that were tested. Antimicrobial activity was retained over a range of pHs (2–11) and temperatures (10–121°C x 15 mins). The draft genome sequence of S. capitis CIT060 was determined and the genes predicted to be involved in the biosynthesis of capidermicin were identified. These genes included the predicted capidermicin precursor gene, and genes that are predicted to encode a membrane transporter, an immunity protein and a transcriptional regulator. Homology searches suggest that capidermicin is a novel member of the family of class II leaderless bacteriocins.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0223541en
dc.identifier.citationLynch, D., O’Connor, P. M., Cotter, P. D., Hill, C., Field, D. and Begley, M. (2019) 'Identification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitis', PLOS ONE, 14(10), e0223541. (17pp.) doi: 10.1371/journal.pone.0223541en
dc.identifier.doi10.1371/journal.pone.0223541en
dc.identifier.eissn1932-6203
dc.identifier.endpage17en
dc.identifier.issued10en
dc.identifier.journaltitlePLOS Oneen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9297
dc.identifier.volume14en
dc.language.isoenen
dc.publisherPLoSen
dc.rights©2019 Lynch et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCapidermicinen
dc.subjectBacteriocinen
dc.subjectStaphylococcus capitisen
dc.subjectCoagulase negative staphylococci (CoNS)en
dc.titleIdentification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitisen
dc.typeArticle (peer-reviewed)en
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